摘要
α-乳清蛋白和β-乳球蛋白系由牛乳清提取的乳糖制品中的主要蛋白杂质,是引起食物及药物过敏的主要过敏原。因此,在食品安全及医药安全领域,对二者的分离及痕量检测是至关重要的。本研究建立了一种简单、快速、灵敏、重现性好的毛细管区带电泳(CZE)方法,使用简单的电泳体系和样品处理步骤,实现了α-乳清蛋白和β-乳球蛋白的基线分离和痕量检测。CZE条件如下:25 mmol/L磷酸盐缓冲液(pH 7.0),分析电压+30 kV,毛细管温度25℃,紫外检测波长205 nm,压力进样5 kPa"10 s。在此条件下,样品分析时间为2 min,α-乳清蛋白和β-乳球蛋白的检出限(LOD)分别为3.0和12 mg/L;迁移时间和峰面积的相对标准偏差(RSD,n=6)分别小于1%和6%,符合实际样品检测要求。本方法已成功用于实际乳糖样品的分析,在相关领域也有很好的应用前景。
α-lactalbumin and fi-lactoglobulin are main impurities in the lactose products extracted from whey, which may cause allergy. Therefore, the separation and detection of race α-lactalbumin and β-lactoglobulin are of great importance to food and pharmaceutical industry. In this work, a simple, fast, sensitive and reproducible capillary zone electrophoresis （CZE） method has been developed for this purpose. After simple sample preparation procedure, α-lactalbumin and fl-lactoglobulin can be baseline separated within 2 minutes under following conditions ： 25 mM phosphate buffer at pH 7.0, ＋ 30 kV applied voltage, 25 ℃ capillary temperature,205 nm UV detection,and sample injection at 50 mbar for 10 so The limit of detection （LOD） at 3 times of S/N is 3.0 and 12 mg/L for α-lactalbumin andβ-actoglobulin, respectively. The repeatability （ RSD, n = 6） is below 1% for migration time and below 6% for peak area. These data qualified the method to the analysis of real lactose samples. The proposed method also shows a great potential in other fields of food and pharmaceutical industry.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2013年第10期1597-1600,共4页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金(Nos.20805001
21175005)
科技部仪器专项(No.2012YQ090194-9)资助项目
