摘要
目的：探讨联合检测外周血单核细胞、中性粒细胞CD64表达水平对感染监测的临床指导意义。方法：随机选取2012年4月～2012年9月在我院就诊的单纯感染性疾病患者90例（其中急性细菌性感染30例，急性病毒性感染30例，慢性乙型肝炎30例）及健康对照者30例，采用流式细胞仪检测外周血单核细胞、中性粒细胞CD64表达水平。结果：单核细胞、中性粒细胞CD64表达水平在急性细菌性感染组（102．16±35．65，22．91±21．66）、急性病毒性感染组（157．10±39．35．24．18±22．95）分别显著高于相应的急性细菌性感染治疗后组（47．22±16．38，4．21±3．69，均P〈0．001）、急性病毒性感染治疗后组（53．47．±14．95，2．72±1．29，均P〈0．001）及健康对照组（39．22±8．77，1．54±0．28，均P〈0．0{31）；单核细胞CD64表达水平在急性病毒性感染组、慢性乙型肝炎组（55．13±16．87）分别高于急性细菌性感染组（P〈0．001）及健康对照组（P〈0．001）。单核细胞与巾性粒细胞CD64比值在急性病毒性感染组（13．96±14．59）、慢性乙型肝炎组（32．78±8．33）也分别高于急性细菌性感染组（6．23±3．10，P=0．008）及健康对照组（26．02±6．75，P=0．001）；急性细菌性感染时中性粒细胞CD64表达水平与m清PCT水平成正相关（r=0．762，P〈0．001）。结论：急性细菌性感染患者中性粒细胞CD64较单核细胞CD64表达明显上调．但其并不能用于区别细菌性或病毒性感染：单核细胞CD64可作为敏感、通用的感染监测指标．其在病毒性感染时上调幅度最大。联合检测单核细胞、中性粒细胞CD64对临床感染的监测及评价抗感染治疗效果有着积极的指导意义。
Objective ： To investigate the clinical significance of monitoring infection, by detecting the expression levels of mon- oc, yte and neutrophil CD64 in peripheral blood. Methods： 90 patients who were hospitalized or followed-up from April 2012 to Septem- ber 2012 in our hospital, including 30 acute bacterial infection patients, 30 acute viral infection patients and 30 chronic hepatitis B （CHB） patients, were enrolled this study. And 30 healthy individuals were taken as healthy controls （HC）. The expression levels of monocyte and neutrophil CD64 in the peripheral blood were detected by flow cytometry. Results： The monocyte and neutrophil CD64 levels in acute bacterial infection group （ 102.16 ± 35.65, 22.91 ± 21.66） and acute viral infection group （ 157.10 ± 39.35, 24.18 ± 22.95 ） were significantly higher than that in the HC group （39.22 ± 8.77, 1.54 ± 0.28, all P 〈 0. 001 ）. After effectively treatment, the monocyte and neutrophil CD64 levels were decreased in acute bacterial infection group （47.22 ± 16.38, 4.21 ± 3.69, all P 〈 0. 001 ） and acute viral infection group （53.47 ± 14.95, 2.72 ± 1.29, all P 〈0. 001 ） compared to before treatment. The level of monocyte CD64 in the acute viral infection group and CHB group （55.13 ± 16.87） was higher than in the acute bacterial infection group （ P = 0.01 ） and HC group （ P 〈 0.01 ）, respectively. The ratio of monocyte CD64 to neutrophil CD64 in the acute viral infection group （13.96 ± 14.59） and CHB group （32.78 ± 8.33） were increased compare to acute bacterial infection group （6.23 ± 3. 10, P = 0. 008 ） and HC group （26.02 ± 6.75, P = 0. 001 ）, respectively. The neutrophil CD64 level was positively correlated with procal- citonin （PCT） （r =0. 762, P 〈 0. 001 ） in acute bacterial infection group. Conclusion： The neutrophil CD64 level was obvious in- creased compare to monocyte CD64 level in the acute bacterial infection patients, but it didn＇t distinguish between bacterial
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2013年第10期1072-1076,共5页
Chinese Journal of Immunology
基金
福建省医学创新课题(2012-CX-33)
泉州市科技计划项目资助课题(2011Z63)
