摘要
目的:提取分离墓头回木质素类化学成分,研究墓头回木脂素类成分的细胞毒活性。方法:采用硅胶柱层析、制备薄层层析、高效液相柱层析等方法分离、纯化化合物,利用NMR、MS等现代波谱技术解析化合物化学结构;MTT法研究化合物体外细胞毒活性。结果:从墓头回中分离得到3个木脂素类成分,鉴定其为:nortrachelogenin(Ⅰ)、(+)-pinoresinol(Ⅱ)、(+)-lariciresinol(Ⅲ);3个化合物体外对PC-3、SGC、NB-4等肿瘤细胞具有一定的抑制作用。结论:墓头回中存在木脂素类成分,该类成分对PC-3、SGC、NB-4等肿瘤细胞具有抑制作用。
Objective：To study the chemical constituents and the anti - tumor activity of Diversifolious Patrinia Root. Methods ： The constituents were isolated on silica gel , PTLC and HPLC. Their structures were identified by UV,^ 1H - NMR, 13 C - NMR, HSQC, and HMBC. Results ： Three compounds were isolated and identified as nortrachelogenin （ I ）, （ ＋ ） -pinoresinol（ Ⅱ）, （ ＋ ） -lariciresinol（ Ⅲ） ;three compounds had inhibition to human tumor ceils. Conclusion：Ef- fective parts of Diversifolious Patrinia Root has the lignans components, and compounds I , II and In can significantly in- hibit PC - 3, SGC and NB - 4 tumor cells.
出处
《中华中医药学刊》
CAS
2013年第10期2168-2170,共3页
Chinese Archives of Traditional Chinese Medicine
基金
浙江省医药卫生科技计划资助项目(2012KYB137)
浙江省大学生科技创新活动计划项目
关键词
墓头回
木脂素
细胞毒活性
Diversifolious Patrinia Root
lignans
cytotoxic activity
