期刊文献+

2,2’,4,4’-四溴联苯醚对人神经母细胞瘤细胞自噬水平的影响 被引量:2

Effects of PBDE-47 on autophagy level in SH-SY5Y cells
原文传递
导出
摘要 目的探讨2,2’,4,4’-四溴联苯醚(2,2’,4,4’-tetrabromodiphenyl ethers,PBDE-47)对人神经母细胞瘤SH-SY5Y细胞自噬水平的影响。方法取对数生长期的SH-SY5Y细胞,分别以终浓度为0(溶剂对照)、1、5、10μmol/L的PBDE-47染毒处理24 h后,采用透射电子显微镜观察细胞内双层膜自噬体结构;以自噬囊泡示踪剂单丹黄酰尸胺(MDC)染色检测自噬囊泡生成情况;采用Western blotting技术检测自噬相关蛋白LC3、Beclin1、P62的表达水平。结果电镜超微结构观察结果显示,对照组细胞内未见异常变化,1μmol/L染毒组细胞内可见轻微的线粒体扩张但未见明显的双层膜自噬体结构,5μmol/L染毒组细胞内可见明显的线粒体空泡及双层膜自噬体结构,10μmol/L染毒组细胞内观察到的双层膜自噬体结构最多;与对照组相比,5、10μmol/L PBDE-47染毒组MDC荧光强度升高,MDC阳性细胞比例增加;此外,5、10μmol/L PBDE-47染毒组自噬相关蛋白LC3、Beclin1、P62的表达水平与对照组相比均明显升高,且差异具有统计学意义(P<0.05)。结论 PBDE-47可诱导SH-SY5Y细胞自噬水平的增加。 Objective To explore the effects of 2, 2', 4, 4' -tetrabromodiphenyl ethers (PBDE-47) on autophagy level in human neuroblastoma SH-SYSY cells. Methods Exponentially growing ceils were incubated with PBDE-47 at the doses of 0 (DMSO control), 1, 5 and 10 μ mol/L respectively for 24 h in vitro. Uhrastructure of autophagosomes, monodansylcadaverine (MDC)-staining autophagic vesicles and the expression levels of autophagy relevant proteins of LC3, Beclinl and P62 were determined by transmission, electron microscope, fluorescence microscopy and Western blotting. Results The results suggested that ultrastrncture of SH-SY5Y ceils were obviously changed by forming autophagosomes with double membrane, along with dilation and degeneration of mitochondria in the 5 and 10 pLmol/L PBDE-47-treated groups compared with the control group, and 10 μmot/L PBDE-47 -treated group ceils could be observed within the most; 5 and 10μmol/L PBDE-47 -treated groups showed an increased relative fluorescence intensity of MDC, namely MDC-positive cell ratio increased. Meanwhile the expression levels of LC3, Beclinl, and P62 protein in 5, 10 μmol/L PBDE-47-treated groups were remarkably increased compared with controls (P〈0.05). Conclusion PBDE-47 may increase the level of autophagy in SH-SY5Y.
出处 《环境与健康杂志》 CAS CSCD 北大核心 2013年第9期755-758,F0003,共5页 Journal of Environment and Health
基金 国家自然科学基金面上项目(81273021 81072266) 唐山市科技计划项目(1313021072)
关键词 2 2’ 4 4’-四溴联苯醚 人神经母细胞瘤SH—SY5Y细胞 自噬 2, 2', 4, 4'-tetrabromodiphenyl ethers Human neuroblastoma SH-SYSY cells Autophagy
  • 相关文献

参考文献17

  • 1Costa LG, Giordano G. Developmental neurotoxicity of polybrominated diphenyl ether (PBDE) flame retardants [J]. Neurotoxicology, 2007, 28: 1047- 1067. 被引量:1
  • 2张琳,田英,杨先丰,崔昌,高宇,王筱金,王沛,丁问微,施蓉,王英,金军,金萍.上海市某医院新生儿脐带血中多溴联苯醚水平及其对出生结局的影响[J].中华预防医学杂志,2011,45(6):490-493. 被引量:12
  • 3Gouin T, Harrier T. Modelling the environmental fate of the polybrominated diphenyl ethers[J]. Environ Int, 2003, 29:717 - 724. 被引量:1
  • 4Herbstman JB, Sj O Din A, Kurzon M, et al. Prenatal exposure to PBDEs and neurodevelopment [J]. Environ Health Perspect, 2010, 118: 712-719. 被引量:1
  • 5陈义虎,姜春阳,吴雪,张舜,张小非,夏涛,王爱国.2,2’,4,4’-四溴联苯醚对人神经母细胞瘤SH-SY5Y细胞胞内游离钙离子浓度的影响[J].环境与健康杂志,2011,28(8):659-662. 被引量:1
  • 6He P, He W, Wang A, et al. PBDE-47-induced oxidative stress, DNA damage and apoptosis in primary cultured rat hippocampal neurons[J]. Neurotoxicology, 2008, 29: 124-129. 被引量:1
  • 7Jiang C, Zhang S, Xia T, et al. The role of the IRE1 pathway in PBDE- 47-induced toxicity in human neuroblastoma SH-SY5Y ceils in vitro [J]. Toxicol Lett, 2012, 211:325 - 333. 被引量:1
  • 8Kim R, Emi M, Tanabe K. Role of mitochondria as the gardens of cell death[J]. Cancer Chemother Pharmacol, 2006, 57: 545-553. 被引量:1
  • 9Ptak G, Zacchini F, Czernik M, et al. A short exposure topolychlorinated biphenyls deregulates cellular autophagy in mammalian blastocyst in vitro[J]. Hum Reprod, 2012, 27: 1034- 1042. 被引量:1
  • 10Glick D, Barth S, Macleod K F. Autophagy: cellular and molecular mechanisms[J]. J Pathol, 2010, 221: 3-12. 被引量:1

二级参考文献13

  • 1Costa LG, Giordano G. Developmental neurotoxieity of polybrominated diphenyl ether (PBDE) flame retardants[J]. Neurotoxieology, 2007,28: 1047-1067. 被引量:1
  • 2Hires RA. Polybrominated diphenyl ethers in the environment and in people:a meta analysis of concentration [J]. Environ Sci Technol, 2004,38 : 945-956. 被引量:1
  • 3Sjodin A, Jones RS, Focant JF, et al. Retrospective time trend study of polybrominated diphenyl ether and polybrominated and polychlorinated biphenyl levels in human serum from the United States [Jl. Environ Health Perspect, 2004,112 : 654-658. 被引量:1
  • 4Costa LG, Giordano G. Developmental neurotoxicity of polybrominated diphenyl ether (PBDE) flame retardants[ J ]. Neurotoxicology, 2007,28 : 1047-1067. 被引量:1
  • 5He W, He P, Wang A, et al. Effects of PBDE-47 on cytotoxicity and genotoxicity in human neuroblastoma cells in vitro [J]. Mutat Res, 2008,649 : 62-70. 被引量:1
  • 6He P, He W, Wang A, et al. PBDE-g7-induced oxidative stress, DNA damage and apoptosis in primary cultured rat hippocampal neumns[J]. Neurotoxicology, 2008,29 : 124-129. 被引量:1
  • 7Hajnoczky G, Davies E, Madesh M. Calcium signaling and apoptosis [ J ]. Biochem Biophys Res Commun, 2003,304 : 445-454. 被引量:1
  • 8Ravagnan L,Roumier T,Kroemer G.Mitochondria,the killer organelles and their weapons[ J ]. J Cell Physiol, 2002,192 : 131-137. 被引量:1
  • 9Wiegand H, Desaiah D,Dehnhardt M,et al. Polyhalogenated hydrocarbon induced perturbation of intracellular calcium homeostasis from astrocytes to human macrophages [J]. Organohalogen Compd, 2001,53 : 182-185. 被引量:1
  • 10Yu K, He YH, Yeung LWY, et al. DE-71 -induced apoptosis involving intracellular calcium and the bax-mitochondria-caspase protease pathway in human neuroblastoma cells in vitro [J-. Toxicol Sci, 2008,104:341-351. 被引量:1

共引文献11

同被引文献7

引证文献2

二级引证文献3

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部