摘要
随着高通量RNA测序(RNA-Seq)技术的发展和测序成本迅速下降,RNA-Seq技术已经成为生物学研究的重要工具,为生物学家全面地了解和研究转录组提供了机遇。高通量测序具有读长短、存在一定比例的测序错误、数据量大等特点,因此RNA-Seq数据分析与基因组分析和传统的EST数据分析有所不同。本文通过介绍不同的测序平台、原始数据产生和低质量数据过滤的计算流程,对短序列比对、转录组拼接、功能注释、以及差异表达分析进行了研究和分析,最后对RNA-Seq在昆虫学研究中的应用进行了综述,并对RNA-Seq技术进行了总结和展望。
With the rapid development of highthroughput RNA sequencing （RNASeq） technology and the rapidly decreasing cost of this method, RNASeq is becoming an important tool for biological research, especially investigating gene function at the transcriptome level. RNASeq typically reads sequences rapidly with a certain percentage of sequence errors and bias producing a huge amount of data. RNASeq data analysis faces lots of challenges. Here, we describe different RNA sequencing platforms, raw data generation processes and data filtering and introduce short sequence alignment, transcriptome assembly, functional annotation and gene expression analysis. Finally, we briefly review the application of RNASeq in insects. The prospects of RNASeq techniques and their application are also discussed.
出处
《应用昆虫学报》
CAS
CSCD
北大核心
2013年第5期1458-1468,共11页
Chinese Journal of Applied Entomology
基金
国家自然科学基金(31171843)
国家高技术研究发展计划("863"计划)(2012AA101505)
关键词
高通量RNA测序
段序列比对
转录组拼接
基因功能注释
基因表达定量
基因差异表达
high-throughput RNA sequencing, short sequence alignment, transcriptome reconstruction, gene function annotation, gene expression quantification, gene differential expression
