摘要
目的 拟制定适合本实验室血细胞仪器分析后血涂片复审准则,以保证血细胞分析结果的准确性和可靠性,更有利于临床对疾病的诊断和治疗.方法 在雅培CELL-DYN3700五分类血细胞计数仪上检测1486份静脉血,先以“国际血涂片复审准则”为标准,记录仪器检测所得各项数据、报警类别和显微镜下所见.结合本实验室实际情况和仪器性能特点,制定适合的血涂片复审准则,并以200份标本验证.结果 以“41条规则”复审,1486份标本真阴性60.4％、真阳性20.8％、假阴性3.0％、假阳性15.8％.以本实验室血涂片复审准则验证200份标本,真阴性69.0％、真阳性18.5％、假阴性2.5％、假阳性10.0％,具有诊断意义的重要参数（幼稚细胞）无漏检.复检率由于36.6％下降到达28.5％.结论 血细胞仪器分析具有局限性,对于形态结构异常的细胞不能作出准确的判断和定位,实验室应根据自身实际情况制定合适的血涂片复审准则,以保证血细胞分析结果的准确性和可靠性.
Objective To ensure the accuracy and reliability of the results of blood cell analysis and help clinical diagnosis and treatment making reexamination criteria for blood smear analyzed by blood cell analyzer in our laboratory.Methods 1486 blood samples were detected by Abbott CELL-DYN3700 hematology analyzer.The data detected by the equipment,the alarm-giving class,and microscopic findings were recorded according to the "international criteria for blood smear reexamination".Appropriate criteria for blood smear reexamination were established in terms of the actual situation and performance characteristics of the instruments in our laboratory.The criteria were verified using 200 specimens.Results The reexamination according to "41 rules" showed that the percentages of true negative,true positive,false negative,and false positive results of the 1486 specimens were 60.4％,20.8％,3.0％,and 15.8％,respectively and were 69.0％,18.5％,2.5％,and 10.0％,respectively after being verified using 200 specimens.The important parameter (immature cells) with diagnostic significance hadn' t been missed.The reexamination rate dropped from 36.6％ to 28.5％.Conclusions Blood cell analyzer can' t make accurate judgment and location for cells with abnormal morphology.Therefore,the laboratory should establish some appropriate reexamination criteria for blood smear according to actual situation to ensure the accuracy and reliability of the results of blood cell analysis.
出处
《国际医药卫生导报》
2013年第19期3032-3035,共4页
International Medicine and Health Guidance News
