摘要
以转赖氨酸基因小鼠为研究对象,根据转入基因的载体结构分别设计赖氨酸基因、新霉素抗性基因、转基因启动子及小鼠基因组内参基因特异性引物,优化反应条件,建立转基因小鼠多重PCR检测方法。结果表明,建立的方法可以用于转赖氨酸基因小鼠的检测,同时为转基因动物检测标准的建立提供试验依据。
By simultaneously amplifying more than one locus in the same reaction,multiplex PCR is becoming a rapid and convenient screening assay.In order to develop the typical testing standard and technical support for transgenic animals and its derived food,a rapid multiplex PCR method for detection of the transgenic mice was established in this study.According to the structure of the transgenic vector,the primers of endogenous gene lysine-rich gene（LR）,marker gene（Neo）,goat promoter（β-casein）and the mouse genome inner control（β-actin,）were designed to establish multiplex PCR detection method in transgenic mice with lysine-rich gene.Results showed that the method described here in which only one reaction was necessary to detect multiple target sequences could be reliably used for the identification of transgenic mice with lysine-rich gene.This work lays the foundation for the detecting standard of transgenic animals.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2013年第9期1403-1406,1416,共5页
Chinese Journal of Veterinary Science
基金
国家转基因生物新品种培育重大专项基金资助项目(2009ZX080072004B)
