摘要
应用RAPD分子标记对日本兵库县 2 0个酒米品种的类群进行了研究 .筛选出对不同基因型酒米品种DNA扩增具有较好多态性的引物 11个 ,它们分别是A0 2 、A0 8、B18、C15、D0 3 、D0 8、G0 5、M11、Q16、S13 和T0 6,根据这些随机引物对不同品种总DNA具有特异性的扩增谱带 ,建立不同品种的RAPD多态性标准模式图 ,可以快速鉴别品种和进行纯度分析 ;依据扩增谱带建立 0、1型数据 ,计算 2 0个酒米品种间的遗传距离 ,以此进行聚类 ,结果表明 ,2 0个酒米品种可被划分为 4大类群 ,划分结果与品种系谱较为一致 。
Heredity groups were classified by cluster analysis with RAPD technology based on 20 rice varieties of hyogo prefecture of Japan. 11 primers, A 02 , A 08 , B 18 , C 15 , D 03 , D 08 , G 05 , M 11 , Q 16 , S 13 and T 06 , selected from 220 candidate primers act as polymorphic DNA (RAPD) makers for 20 rice. The maps of RAPD standard polymorphic DNA in different varieties can be established by their specific characteristic bands. Thus, the cultivars and their purity could be identified and analysed quickly. In addition, Genetic distance among 20 rice varieties was calculated and clustered with RAPD makers. The results showed that 20 rice varieties could be classified into four groups. The four groups classified by cluster analysis with RAPD technology is consistent with the expected grouping based on available pedigree. The current study indicated that it was practical to group rice with RAPD technology.
出处
《华南农业大学学报》
CAS
CSCD
北大核心
2000年第4期4-6,共3页
Journal of South China Agricultural University
基金
日本兵库县农业尖端技术项目
