摘要
目的建立猪肉中氯丙嗪残留量的气相色谱质谱测定方法。方法样品经乙酸乙酯提取,乙腈正己烷分配脱脂,MCS固相萃取柱净化,5%氨化甲醇洗脱,以气相色谱质谱选择86、233、272、318碎片离子进行定性定量分析。结果氯丙嗪定量分析在0.051～0.25μg/ml呈良好的线性关系,r=0.999 2,检出限为2.0μg/kg,平均回收率为87.1%～97.3%,RSD为3.15%。结论该方法适合猪肉中氯丙嗪的检测。
Objective To establish a method for the determination of chlorpromazine in pork by gas chromatography-mass spectrometry.Methods The sample was homogenized and extracted by ethyl acetate,degreased by hexane and acetonitrile,purified by solid phase extraction,and eluted by 5% ammoniated methanol.Ion fragments of 86,233,272 and 318 were selected for qualitative and quantitative analysis by gas chromatography-mass spectrometry.Results Good linearities were shown in 0.051-0.25 μg/ml with the relative coefficients of 0.999 2.The limit of detection was 2.0 μg/kg.The average recoverys were from 87.1% to 97.3% and the relative standard derivation was 3.15%.Conclusion It could be applied to determine the chlorpromazine in pork.
出处
《中国食品卫生杂志》
北大核心
2013年第5期438-440,共3页
Chinese Journal of Food Hygiene
关键词
气相色谱质谱法
氯丙嗪
猪肉
兽药残留
Gas chromatography-mass spectrometry
chlorpromazine
pork
veterinary drug residue
