摘要
目的 探讨胺基化碳纳米管携带质粒转染细胞并获得稳定转染的可行性,同时验证其生物安全性.方法 胺基化碳纳米管携带质粒pcDNA-GFP-N1转染中国仓鼠卵巢（CHO）细胞,48 h后荧光显微镜下确认转染成功,加入G418（ll00 mg/L）筛选稳定转染细胞；采用XTT法测定胺基化碳纳米管的细胞毒性.结果 胺基化碳纳米管携带质粒成功转染CHO细胞；通过G418筛选2周,最终获得稳定转染细胞.胺基化碳纳米管无明显的细胞毒性.结论 胺基化碳纳米管可以携带外源基因进入细胞,并且可以将其整合到宿主的染色体进行稳定转染.胺基化碳纳米管的生物安全性较好.
Objective To explore the feasibility of using the amino-carbon nanotubes as gene vector to stably transfect cells,and to identify the safety of amino-carbon nanotubes as biomedical material.Methods The amino-carbon nanotubes were used as gene vector carrying plasmid pcDNA-GFP-N1 to transfect Chinese hamster ovary （CHO） cells.After successful transfection was confirmed under fluorescence microscopy at 48 h,the G418 （1100 mg/L） was added to select stably transfected cells.The cytotoxicity of amino-carbon nanotubes was determined by using XTT methods.Results The successful transfection in CHO cells was accomplished using amino-carbon nanotubes as the plasmid vector.Furthermore,the stably transfected cells could be eventually obtained two weeks after G418 was added.The amino-carbon nanotubes didn＇t have obvious cytotoxicity.Conclusion Amino-carbon nanotubes could not only transport genes into cells and accomplish the transient transfection,but also carry genes into the nucleus,integrating the foreign genes into the host chromosome to achieve stable transfection.Amino-carbon nanotubes were safe as biomedical materials.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2013年第9期1867-1868,共2页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金资助项目（81071884）
教育部博士点基金资助项目（20110071110065）
关键词
胺基化碳纳米管
转染
细胞毒性
Amino-carbon nanotube
Transfection
Cytotoxicity
