摘要
为研究饲料中添加不同水平的维生素E(VE)对急性热应激及常温恢复条件下团头鲂血清生理生化、激素及肠道抗氧化指标的影响,实验挑选180尾健康、规格一致的团头鲂(45.00±0.50)g,分成3组,每组4个平行,每组分别添加不同量维生素E(0、50和400 mg/kg饲料)。饲养90 d后,对团头鲂进行32℃应激1 d,并在25℃常温恢复7 d。结果表明:应激前,50 mg/kg VE组显著提高了肠道谷胱甘肽的活性(P<0.05);高温应激后,与对照组相比,50 mg/kg VE组在高温应激1 d后显著降低了谷丙转氨酶和皮质醇含量,常温恢复7 d后显著降低了谷草转氨酶、皮质醇的含量(P<0.05),显著提高了碱性磷酸酶、谷胱甘肽和超氧化物歧化酶活性(P<0.05);与对照组比,400 mg/kg VE添加组在32℃应激1 d及25℃恢复1 d后显著降低了谷丙转氨酶活性(P<0.05),常温恢复7 d后显著降低了皮质醇浓度,在应激1 d及恢复7 d后均显著降低了丙二醛浓度(P<0.05),400 mg/kg VE添加组在高温应激1 d后显著提高了碱性磷酸酶活性和三碘甲腺原氨酸含量,常温恢复7 d后显著提高了碱性磷酸酶活性(P<0.05)。因此,添加适量的维生素E(50～400 mg/kg)有利于调节血脂变化,提高团头鲂肠道抗氧化能力,并且可缓解高温应激对团头鲂血液指标波动的影响,减轻脂质过氧化水平,对团头鲂起到一定保护作用。
The purpose of this study was to investigate the effects of vitamin E（VE）on serum biochemical indices and antioxidant capacity of juvenile Megalobrama amblycephala under acute high temperature stress and recovery.180 healthy fish（45.00±0.50）g were selected and randomly divided into three groups：a control group was fed with basal diet,and two treatment groups fed with basal diet supplemented with 50 and 400 mg/kg VE,respectively.Each group has four replications.After 90 days’ feeding trial,all fish were exposed to high temperature stress（32 ℃）for 1 day and recovered at 25 ℃ for 7days in a controlled temperature aquarium.The results showed that the diet supplemented with 50 mg/kg VE significantly increased the activity of intestine glutathione compared with the control group（P＆lt;0.05）prior to the stress.After high temperature stress,the diet supplemented with 50 mg/kg VE significantly decreased the levels of serum aspertate aminotransferase（ALT）activity and cortisol（COR）content at 1d after stress,reduced the levels of serum alanine aminotransferase（AST）activity,triiodothyronine（T3）and COR content at 7 d recovery after stress（P＆lt;0.05）,and improved the serum alkaline phosphatase（ALP）activity,intestine glutathione（GSH）activity and superoxide dismutase（SOD）activity at 7d recovery after stress（P＆lt;0.05）compared with the control group（P＆lt;0.05）.The diet supplemented with 400 mg/kg VE significantly reduced the levels of serum ALT and intestine malonaldehyde（MDA）content at 1 day after stress and 7 d recovery after stress,COR content at 7 d recovery after stress（P＆lt;0.05）and increased the levels of serum ALP and T3 at 1 day after stress concentration compared with the control group（P＆lt;0.05）.So it is suggested that ingestion of a basal diet supplemented with VE（50-400 mg/kg diet）could help regulate the change of blood lipids,improve antioxidant capacity,alleviate the change of blood biochemical parameters in high temperature
出处
《水产学报》
CAS
CSCD
北大核心
2013年第9期1369-1377,共9页
Journal of Fisheries of China
基金
公益性行业(农业)科研专项(201003020)
现代农业技术体系建设专项(CARS-46)
中央级公益性科研院所基本科研业务费专项(2012A0602)
关键词
团头鲂
维生素E
热应激
生化指标
抗氧化
Megalobrama amblycephala
vitamin E
high temperature stress
biochemical indices
antioxidant capacity
