摘要
目的 研究C/EBP同源蛋白（C/EBP homology protein,CHOP）在严重创伤性脑损伤（traumatic brain injury,TBI）患者颅脑周围组织中的表达,探讨其与损伤严重程度的关系. 方法 选取41例TBI患者（TBI组）颅脑周围组织,并选取因其他疾病（排除TBI及其他中枢神经系统疾病）死亡的16例尸检标本作为对照组,对照组及TBI组按年龄分为未成年组（≤18岁）、成年组（18 ～59岁）和老年组（＞59岁）.TBI组患者根据入院时GCS评分再分为重型TBI组（GCS 6 ～8分）和特重型TBI组（GCS 3～5分）.比较不同年龄、性别及GCS组间CHOP在TBI周围组织中的表达差异.TUNEL法检测神经细胞凋亡,并对CHOP表达水平与凋亡数量进行相关性分析. 结果 对照组CHOP表达在年龄与性别间差异无统计学意义（P＞0.05）.与对照组比较,TBI组CHOP表达显著上调（P＜0.05）.在TBI组中,CHOP表达在性别间差异无统计学意义（P＞0.05）,而老年组患者CHOP表达低于成年组和未成年组（P＜0.05）,特重型TBI组明显高于重型TBI组（P＜0.05）.TBI组较对照组神经细胞凋亡数量明显增加（P＜0.05）,且CHOP表达水平与凋亡指数呈正相关（r =0.72,P＜0.05）. 结论 TBI后CHOP表达水平与损伤严重程度及神经细胞凋亡密切相关,但CHOP诱导的凋亡途径可能不是老年组TBI后继发性脑损害的主要因素.
Objective To investigate the expression of C/EBP homology protein （CHOP） in peripheral brain tissue of patients with severe traumatic brain injury （TBI） and its correlation with the injury severity.Methods The study included peripheral brain tissues of 41 TBI patients （TBI group）.Another 16 autopsy specimens succumbed to other diseases （except for TBI or other central nervous system diseases） were selected as controls.The control group and TBI group were subdivided into immaturity group （≤18 years）,adult group （18-59 years） and elderly group （〉59 years）.According to Glasgow Coma Scale （GCS） on admission,TBI group was classified as severe TBI group （GCS of 6-8） and particularly-severe TBI group （GCS of 3-5）.CHOP expression in peripheral tissues after TBI was compared in between different age,gender and GCS.Nerve cell apoptosis was detected by TUNEL technique and correlation between CHOP level and apoptotic number was analyzed.Results There were no age and gender differences regarding CHOP expression in control group （P 〈 0.05）.Compared with control group,expression of CHOP presented notable up-regulation in TBI group （P 〈 0.05）.Expression of CHOP presented no gender difference in TBI group （P 〉 0.05）,but its expression was lower in the aged than in adult or immaturity （P 〈 0.05） as well as notably higher in particularly-severe TBI group than in severe TBI group （P 〈 0.05）.Nerve cell apoptosis in TBI group was far greater in number than that in control group （P 〈0.05）.A positive correlation was observed between CHOP level and apoptotic index （r =0.72,P 〈 0.05）.Conclusion Expression level of CHOP after TBI is closely related to the injury severity and nerve cell apoptosis,but the apoptosis pathway induced by CHOP may not be a major factor in secondary brain injury after TBI in the aged patients.
出处
《中华创伤杂志》
CAS
CSCD
北大核心
2013年第9期820-823,共4页
Chinese Journal of Trauma
基金
国家自然科学基金面上资助项目（30973087）
国家临床重点专科建设资助项目（财社[2011]170号）
关键词
脑损伤
凋亡
C
EBP同源蛋白
Brain injuries
Apoptosis
C/EBP homology protein
