摘要
目的观察不同浓度二甲双胍对体外培养人成骨细胞增殖、CollagenⅠ及LRP5mRNA表达的影响,探讨二甲双胍对成骨细胞作用的可能机制。方法不同浓度二甲双胍(0、25、50、100、200μmol/L)刺激体外培养人成骨细胞72 h,采用CCK-8比色法检测细胞增殖情况,用荧光定量RT-PCR法检测CollagenⅠ、LRP5mRNA表达。结果显示二甲双胍可促进其增殖(P<0.05),并促进成骨细胞CollagenⅠ及LRP5mRNA表达(P<0.05),在200μmol/L时增殖、表达最明显,呈剂量效应关系。结论二甲双胍在一定浓度范围内可促进人成骨细胞增殖,可能通过Wnt/LRP5信号通路增强CollagenⅠ及LRP5mRNA的表达来调控。
Objective To observe the effect of different concentrations of metformin on the proliferation of human osteoblasts and the mRNA expression of collagen I and LRP5, and to investigate the possible mechanism of the effect of mctformin on human osteoblasts. Methods Human osteoblasts cultured in vitro were treated with different concentrations of metformin （0, 25, 50, 100, and 200 μmol/L） for 72 hours. Then cell proliferation was assessed with CCK-8 eolorimetric method. The mRNA expression of collagen I and LRP5 was detected using fluorescent quantitative RT-PCR. Results Metformin could promote the proliferation of osteoblasts （P 〈 0.05） , and also could promote the mRNA expression of collagen I and LRP5 （P 〈 0.05 ）. The promotion effect was highest at the concentration of 200μmol/L, showing a dose-dependent manner. Conclusion In a certain range of concentration, metformin can promote the proliferation of human osteoblasts. It may regulate this progress by enhancing the mRNA expression of collagen I and LRP5 through the Wnt/LRP5 signal pathway.
出处
《中国骨质疏松杂志》
CAS
CSCD
北大核心
2013年第9期934-937,共4页
Chinese Journal of Osteoporosis
