摘要
目的:观察阿胶补肾健骨方对去卵巢大鼠骨质疏松症(OP)的影响,探讨阿胶补肾健骨方防治OP的作用机制。方法:将70只SD大鼠随机分为假手术组(J组)、模型组(M组)、阿胶补肾健骨方组(E组)和阿仑膦酸钠组(A组)。各组手术伤口恢复后,第7天开始灌胃(除J组外)治疗3个月,于末次给药后24 h处死大鼠并采集标本。采用ELISA法检测各组大鼠血清中OCN、PICP水平。结果:M组于造模后2个月起,体重明显高于J组(P<0.05);E组、A组体重渐增,显高于J组(P<0.05);M组、E组、A组大鼠子宫、双肾指数均不同程度均低于J组(P<0.05);E组、A组大鼠子宫、双肾指数均高于M组(P<0.05);E组大鼠子宫、双肾指数优于A组(P<0.05);M组大鼠血清OCN、PICP均明显低于J组(P<0.05);E组、A组大鼠血清OCN、PICP水平均高于M组(P<0.05);A组大鼠血清OCN高于E组(P<0.05);E组大鼠血清PICP高于A组(P<0.05)。结论:阿胶补肾健骨方可调节脂代谢,升高OP大鼠子宫及双肾指数;升高OP大鼠血清中OCN和PICP的水平,从而达到预防和治疗OP的目的。
Objective： To observe effects of Ejiao Bu Shen Jian Gu Formula on rats which were completely removal of ligation and ovariectomy; to explore the action mechanism of preventing and treating osteoporosis of Ejiao Bu Shen Jian Gu Formula. Methods： Seventy female SD rats, postmenopausal osteoporotic animal model were established. Rats were randomly di- vided into sham-operated group, model group, group A of osteoporotic animal model with Alendronate sodium and group E of osteoporotic animal model with Ejiao Bu Shen Jian Gu Formula. When the operative wound recovered, the model group, group A and group E were given a gavage （except sham-operated group） for three months. Rats were killed and collected specmen after 25 hours when the rats were given a gavage last time. The serum OCN,the PICP were detected by enzyme-linked im- munosorbent assay（ELISA） in rats. Results： The changes of weight of rats in group M was significantly higher than the sham- operated rats after 2 months（P〈0.05）; The weight of rats in group E and in group A was gradually increased and was signifi- cantly higher than the sham-operated rats（P〈0.05）; Index of uterus, both kidneys of rats in group E,group A and group M were lower than the sham-operated rats In varying degrees （P〈0.05）; Index of uterus, both kidneys of rats in group E,group A were higher than group M （P〈0.05）; Index of uterus, both kidneys of rats in group E were better than group A （P〈0.05）; The serum OCN and PICP of group M were significantly lower than group J（P〈0.05）; The serum OCN and PICP of group E and group A were significantly higher than group M（P〈0.05）; The serum OCN of group A was higher than group E（P〈0.05）; The PICP of group E was higher than group A（P〈0.05）. Conclusion： Ejioo Bu Shen Jian Gu Formula can adjust the lipid metabolism, increase index of uterus and kidneys and improve the level of OCN and PICP. There was a better prevention and treatment that Ejiao Bu Shen Jian Gu Formula can be treated for
出处
《中医药导报》
2013年第9期66-69,共4页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
