摘要
目的研究肝纤维化进程中大鼠肝脏血管紧张素转换酶(ACE)、血管紧张素转换酶(2ACE 2)水平及ACE/ACE 2的动态变化。方法制备四氯化碳(CCl4)诱导大鼠肝纤维化模型,分别于造模第15、30、45、60天处死模型组大鼠各6只,各组大鼠肝组织行HE和Masson染色,Western blot法检测肝组织匀浆ACE、ACE2蛋白表达。结果在大鼠肝纤维化过程中,肝组织ACE、ACE 2水平呈上升趋势;第15、30天模型组ACE/ACE 2比值较对照组降低(P<0.05),而第45天模型组其比值高于对照组(P<0.05),第60天时该比值明显增高(P<0.01)。结论 ACE、ACE 2均参与大鼠肝纤维化形成。
Objective To explore variation on level of angiotensin converting enzyme（ACE） and angiotensin converting enzyme 2（ACE 2）, ratio of ACE/ACE 2 in hepatic fibrosis in rats. Methods Except rats in control group,all were given subcutaneous injection of 40% CC14. Each 6 rats in the model group were killed at 15, 30, 45 and 60 day respectively. Histopathological study of liver tissue was done with hematoxylin-eosin（HE）and Masson trichrome staining.ACE arid ACE 2 levels were determined by Western blot analysis. Results The level of ACE and ACE 2 show ascendant trend during fibrosis rats, ACE^ACE 2 ratio of model group at 15, 30 day reduced than control group. Conclusion The occurrence and development of hepatic fibrosis are possibly associated with imbalances between ACE and ACE 2.
出处
《当代医学》
2013年第28期22-24,共3页
Contemporary Medicine
