摘要
目的探究BAY(激素敏感脂酶抑制剂)及DMSO(二甲基亚砜)对小鼠精子顶体反应(AR)的直接影响。方法分别通过5个和6个不同的浓度梯度的BAY及DMSO在体外探究其对小鼠精子AR的影响,在不同孵育时间段采用FITC-PSA和考马斯亮蓝染色法对小鼠精子的顶体反应进行鉴定。结果在所选浓度范围内BAY未能直接影响精子的顶体反应及存活率(P>0.05),而DMSO在一定浓度和时间内可直接影响精子顶体反应及精子存活率。结论 DMSO和BAY对小鼠精子发生的作用是通过不同途径来实现的,前者可直接影响精子顶体反应和精子的存活率,而后者则与顶体反应及存活率无关。提示在AR的研究中使用DMSO时需控制好作用浓度及作用时间。
Objective The effects of BAY and DMSO on acrosome reaction in vitro in mice spermatozoa were investigated.Method The influence of both chemicals on AR in vitro in mice by using 6 different concentrations of DMSO and 5 different concentrations of BAY for 5 duration time were invesgigated.The AR was evaluated by FITC-PSA and Coomassie brilliant blue stain.Result Both sperm AR rate and survival rate varied with could be affected by DMSO within a certain concentration and time,while the BAY had litter influence on them with all measured concentration（P0.05）.Conclusion The results showed that the BAY and DMSO had different pattern on sperms.DMSO has a direct effect on both the AR rate and survival rate,while the influence of BAY on the AR rate and the survival rate is neglectable.Thus a proper usage of DMSO in manipulation of sperm should be carefully reconsidered.
出处
《实验动物科学》
2013年第3期1-5,I0001,共6页
Laboratory Animal Science
基金
国家自然科学基金(No.30870934)
