摘要
目的:观察人慢性牙周炎牙龈组织中缺氧诱导因子1α(HIF-1α)的表达水平,探讨HIF-1α在牙周炎发病中的可能作用。方法:将55例自愿接受研究的牙周炎患者按照牙周炎的病变程度分成3组:正常对照组15例、中度牙周炎组20例和重度牙周炎组20例。取牙龈组织标本,4%中性甲醛液固定48 h以上。制作牙龈组织的连续切片,HE染色,光学显微镜下观察各组牙龈组织的组织学改变;免疫组织化学染色法观察各组牙龈组织HIF-1α的表达水平。结果:(1)与正常对照组相比,各慢性牙周炎组的牙龈组织HIF-1α的表达水平显著升高(P<0.01);(2)重度牙周炎组HIF-1α阳性细胞比例明显高于中度牙周炎组(P<0.05);(3)HIF-1α阳性细胞比例与牙周炎的病变程度呈正相关。结论:人慢性牙周炎牙龈组织中HIF-1α的表达水平随着牙周炎病变程度的加重而升高,提示组织缺氧可能在牙周炎的疾病进程中起着重要的作用。
AIM：To observe the expression of hypoxia-inducible factor 1α （HIF-1α） in human gingival tissues with chronic periodontitis. METHODS：A total of 55 volunteers, including 15 healthy controls, 20 cases of moderate chronic periodontitis and 20 cases of severe chronic periodontitis, were involved in this study, and their gingival specimens were taken and fixed in 4% neutral formalin. The histological changes of gingival tissues were observed by HE staining, and the expression of HIF-1α in gingival tissues was detected by immunohistochemical staining. RESULTS： The proportion of HIF-1α positive cells in gingival tissues was significantly higher in chronic periodontitis groups than that in healthy control group （P〈0.01）, and that in severe chronic periodontitis group was significantly higher than that in moderate chronic periodontitis group （P〈0.05）. There was a significantly positive correlation between the severity of chronic periodontitis and the proportion of HIF-1α positive cells in gingival tissues. CONCLUSION：The expression of HIF-1α in human gingival tissues is increased with the severity of chronic periodontitis, suggesting that hypoxia may play an important role in chronic periodontitis.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2013年第9期1668-1671,共4页
Chinese Journal of Pathophysiology
基金
广东省医学科研基金资助项目(No.A2013454)
关键词
牙周炎
缺氧诱导因子1Α
缺氧
Periodontitis
Hypoxia-inducible factor 1 α
Hypoxia
