摘要
研究Na+,K+ -ATP酶与小型猪复合麻醉剂全麻作用的关系,以判断该酶是否为该制剂作用的靶位之一。试验选取84只SD大鼠,先随机抽取12只为对照组,其余随机均分为高剂量小型猪复合麻醉剂组(腹腔注射(ip)7.5 mg/kg)和低剂量小型猪复合麻醉剂组(5 mg/kg),每个剂量组又随机均分为麻醉组、恢复Ⅰ组和恢复Ⅱ组等3个亚组。对照组注射生理盐水10 mL/kg,5 min后断头取材;麻醉组、恢复Ⅰ组和恢复Ⅱ组分别在翻正反射消失即刻、翻正反射恢复即刻和大鼠可直线爬行后断头取材。迅速分离双侧大脑皮层、海马、小脑、脑干、丘脑,立即液氮冷冻。制备脑粗突触体,采用比色法测定Na+,K+-ATP酶活性。结果表明:2个剂量麻醉组大脑皮层、脑干和丘脑突触体Na+,K+-ATP酶活性受到明显抑制(P<0.01或P<0.05),高剂量组小脑该酶也发生明显变化(P<0.05)。在恢复期上述脑区的该酶活性呈现不同程度恢复,与对照组相比,差异不显著(P>0.05),海马在2剂量组未发生明显的变化。小型猪复合麻醉剂的全麻作用与抑制小脑和丘脑突触体Na+,K+-ATP酶活性相关,说明此酶可能是小型猪复合麻醉剂全麻作用的靶位之一。
The relationship of Na＋ , K＋-ATPase and the anesthetic effect of miniature pig compound anesthetic was investigated to determine whether the enzyme was one of the targets to evaluate the anesthetic effect. In 84 SD rats, 12 were randomly selected for the control group, and the remaining＇were randomly divided into high-dose （ ip, 7. 5mg/kg） and low-dose miniature pig compound anesthetic groups （ ip, 5mg/kg） Each dose group was randomly divided into 3 subgroups ： anesthesia group, recovery I group and recovery 11 group. Control group were sampled after injection （ip） with 10mL/kg of saline for 5 min; anesthesia group, recovery I group and recovery group II were sampled instantly in the loss of righting reflex, the recovery of righting reflex and straight line crawling. Samples included the bilateral cere- bral cortex, hippocampus, cerebellum, brain stem and thalamus, and immediately frozen in liquid nitrogen. Brain synaptosomals were pre- pared and tested for Na~ , K＋-ATPase activities. The results showed that in the two doses of anesthetic groups, Na＋ , K＋-ATPase activities of cerebral cortex, brain stem and thalamus synaptosomes were significantly inhibited （P〈0. 01 or P〈0. 05 ）; the enzyme activity also changed significantly in cerebellum of high dose group （ P〈0. 05 ）. In the recovery period, the enzyme activities in these regions showed varying degrees of recovery, compared with control group, but there were no significant difference （P〉0. 05）. No obvious changes occurred in hippocampus of two dose groups. The miniature pig compound anesthetic effect related with the suppression of Na＋ , K＋-ATPase activities in cerebellum and thalamus synaptosomes. This enzyme may be one of the main targets in the miniature pig compound anesthetic effect.
出处
《畜牧与兽医》
北大核心
2013年第9期31-34,共4页
Animal Husbandry & Veterinary Medicine
基金
国家自然科学基金项目(31272617)
哈尔滨市青年科技创新人才基金项目(2010RFQNX105)
高等学校博士学科点专项科研基金项目(博导类:20102325110003)
