摘要
为发掘与抗白粉病基因紧密连锁的分子标记,以台烟7号为母本,NC89为父本,构建F2群体,利用烟草白粉病致病菌二孢白粉菌(Erysiphe cichoracearum DC)进行人工接种鉴定。筛选2317对SSR引物,得到61对多态稳定的引物,用其对285株F2单株进行分析,构建遗传连锁图谱。利用WinQTLCart 2.5软件对烟草白粉病抗性基因进行QTL定位。检测到1个烟草白粉病抗性基因QTL位点,与SSR引物52725紧密连锁,遗传距离为1.01cM,其加性效应为-14.38,贡献率为21.02%。
Molecular markers closely linked with resistance genes were explored with SSR technology. Population F2 was constructed from resistant variety Taiyan 7 as female parent and susceptible variety NC89 as male parent. An artificial inoculation method was adopted to test degree of the resistance to Erysiphe cichoracearum DC of their F2 family. 61 polymorphic markers obtained by screening 2317 SSR primers in 24 linkage groups were used to analyze F2 population. QTL analysis on powdery mildew resistance was conducted by WinQTLCart 2.5 software. One QTL was located on the 14th linkage group, and tiLghtly linked with maker 52725 with genetic distance of 1.01 cM. The additive effect of QTL was -14.38 and 21.02% ofphenotypic variation can be explained by QTL.
出处
《中国烟草学报》
EI
CAS
CSCD
北大核心
2013年第4期105-108,共4页
Acta Tabacaria Sinica
基金
国家烟草专卖局专项"中国烟草种质资源平台建设"(国烟办综[2005]501号)
全国烟草有害生物调查研究(110200902065)
