摘要
目的探讨发光二极管(LED)红光照射对高脂血症大鼠血脂的调节作用。方法健康雄性Sprague-Dawley大鼠36只随机分为正常对照组(n=12)和高脂模型组(n=24)。正常对照组饲喂普通饲料,高脂模型组采用高脂饲料喂养6周。造模成功后,再将高脂模型组随机分为高脂对照组(n=12)和LED治疗组(n=12)。高脂对照组不给予照射,LED治疗组给予LED红光照射,波长(630±15)nm,每天30 min,连续照射28 d。生化法检测大鼠血清总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL-C)、高密度脂蛋白(HDL-C)水平及脂蛋白酯酶(LPL)和肝脂酶(HL)活性。肝脏组织免疫组化染色观察羟甲基戊二酰辅酶A还原酶(HMG-CR)的表达。结果 LED治疗组大鼠血清TC、TG和LDL-C水平较高脂对照组明显减低(P<0.01),而HDL-C水平明显升高(P<0.01);LED治疗组血清LPL和HL的活性明显高于高脂对照组(P<0.01),LED治疗组肝脏组织中HMG-CR的表达低于高脂对照组(P<0.05)。结论LED红光照射通过增强LPL、HL的活性及降低HMG-CR的表达,影响血清脂质的代谢,从而达到调节血脂的作用。
Objective To observe the effect of light emitting diode （LED） red light irradiation on serum lipid in experimental hyperlipid- emia rats. Methods 36 healthy male Sprague-Dawley rats were randomly divided into normal control group （n=12） and hyperlipidemic mod- el group （n=24）. The normal group was fed with normal diet while the hyperlipidemic model group with fat-rich forage for 6 weeks. The hy- perlipidemic model group rats were randomly divided into the hyperlipidemic control group （n=12） and LED treatment group （n=12）, and the latter accepted LED red light irradiation for 28 d. The levels of serum lipid including total cholesterol （TC）, triglyceride （TG）, low-densi- ty lipoprotein （LDL-C）, high-density lipoprotein （HDL-C）, and the activities of lipoproteinesterase （LPL） and hepatic lipase （HL） were de- tected with biochemical assay. The expression of 3-hydroxy-3-methylglutaryl coenzyme A reducase （HMG-CR） of hepatic tissue were mea- sured with immunohistochemical staining. Results Compared with the hyperlipidemic control group, the levels of serum TC, TG and LDL-C decreased while the serum HDL-C increased significantly in the LED treatment group （P〈0.01） after treated with LED. The levels of LPL and HL in serum increased （P〈0.01） while the activity of HMG-CR decreased （P〈0.05）. Conclusion LED red light irradiation might play a regulating effect on serum lipid by enhancing the activities of LPL and HL and inhibiting the expression of HMG-CR to interfere the metabolism ofTC, TG, LDL-C and HDL-C.
出处
《中国康复理论与实践》
CSCD
北大核心
2013年第9期824-827,共4页
Chinese Journal of Rehabilitation Theory and Practice
关键词
红光照射
高脂血症
脂蛋白酯酶
肝脂酶
羟甲基戊二酰辅酶A还原酶
大鼠
red light irradiation
hyperlipidemia
lipoproteinesterase
hepatic lipase
3-hydroxy -3-methylglutaryl coenzyme A redu-case
rats
