摘要
生物钟节律基因CCA1在植物的光周期反应中起着重要的调控作用。利用RT-PCR方法克隆获得了一条包含1 902 bp开放阅读框的杨树CCA1基因cDNA序列,其编码633个氨基酸残基,蛋白的分子量约为68.90 kDa,等电点为6.33。分析显示含有1个Myb-like DNA结合域及2个蛋白跨膜结合域。实时荧光定量PCR分析发现,PtCCA1基因主要在杨树叶组织中表达;随着光照时间的变化,该基因在杨树中的表达量呈现白昼不断降低而夜晚逐渐升高的昼夜变化趋势。研究结果为进一步探索PtCCA1基因在调控杨树光周期响应途径中的功能以及杨树光周期敏感机制提供了科学基础。
CCA1 gene plays an important role in circadian clock sensitivity in Populus. In this study, PtCCA1 was i- solated from Populus tomentosa by RT-PCR. It has an open reading frame （ORF） of 1902 bp in length and encodes a protein of 663 amino acid residues, with protein molecular weight of 68.90 kDa and isoelectric point of 6.33. The amino acid sequence contained one Myb-like DNA binding domains and two putative transmembrane spanning re- gions. The results of fluorescence quantitative RT-PCR showed that the PtCCA1 gene was expressed mainly in leaf tissues. Along with the change of illumination time, the expression amount of PtCCA1 gene decreased during the day but gradually increased during the night. This study provides some references for further study of PtCCA1 function in regulating photoperiod sensitivity and the mechanism of circadian synchronization in poplars.
出处
《林业科学研究》
CSCD
北大核心
2013年第5期649-654,共6页
Forest Research
基金
国家自然科学基金项目(30571518)
