摘要
报道了青霉素G钠对绿巨人愈伤组织诱导、分化及苗生长的初步结果 .试验表明 :青霉素G钠对诱导叶片愈伤组织的产生有抑制作用 ,而对愈伤组织的分化有促进作用 ,效果最好的浓度为 60万u·L- 1,平均每块愈伤组织可分化 7.0个不定芽 ;低浓度的青霉素G钠 ( 2 0万u·L- 1)对试管苗的生长有促进作用 ,同时也促进根系的发育 ;而高浓度的青霉素G钠对试管苗的生长有抑制作用 ,并随着浓度的提高而增强 ;在生根培养基中添加低浓度的青霉素G钠( 2 0万u·L- 1)比不加青霉素G钠的培养基的试管苗移栽成活率高 .
A preliminary results were reported on the effects of penicillinum G natricum on the induction and differentiation of Spathiphyllum Kochii’s callus tissue,and on its seedling growth.The results showed that penicillinum G natricum has an inhibitory effect on inducing its leaf callus tissue,and has a promotive effect on the differentiation of its callus tissue; for the concentration of penicillinum G natricum 6 000 000 u·L -1 ,7.0 adventitious buds can be differentiated by each callus tissue,with the best effect; the medium with a lower penicillinum G natricum concentration has a promotive effect on the growth of the test tube seedlings and the development of its root system, while the medium with a higher penicillinum G natricum concentration has an inhibitory effect on the growth of the test tube seedlings,and this inhibitory effect become stronger with the increased concentration; for transplantation, the survival rate of test tube seedlings cultivated from the medium added a lower concentration of penicilinum G natricum (2 000 000 u·L -1 )is higher than that cultivated from the medium with no penicillinum G natricum.
出处
《海南大学学报（自然科学版）》
CAS
2000年第4期401-405,共5页
Natural Science Journal of Hainan University
关键词
青霉素G钠
绿巨人
愈伤组织
分化
诱导
抗菌药物
penicillinum G natricum
Spathiphyllum Kochii
callus tissue
differentiation4
