摘要
为给火龙果的功能基因表达和调控研究提供内参基因,根据Actin基因和UBQ基因的保守区设计引物,采用RT-PCR方法克隆火龙果Actin基因和UBQ基因片段。结果表明:克隆的Actin基因和UBQ基因片段大小分别为302bp、192bp,分别编码100个氨基酸和63个氨基酸。Actin基因片段与其他植物Actin基因核苷酸序列的同源性均在78%以上,与氨基酸序列的同源性达88%以上;UBQ基因片段与其他植物UBQ基因核苷酸序列的同源性均在88%以上,与氨基酸序列的同源性达95%以上。
To provide housekeeping genes for studying on the expression and regulation of genes in pitaya, primers was designed according to the conserved sequences of genes Actin and UBQ. The fragments of the two housekeeping genes were obtained by RT-PCR. Results： Actin fragment of pitaya contained 302 bp nucleotide acids, encoding 100 amino acids. Homology comparison with gene Actin sequence from other plants showed that they shared over 78%nucleotide sequence, and 88% amino acid sequence. The UBQ gene fragment contained 192 bp nucleotide acids, encoding 63 amino acids. Homology comparison with gene UBQ from other species demonstrated that they shared over 88% nucleotide sequence, and 95% amino acid sequence.
出处
《贵州农业科学》
CAS
北大核心
2013年第9期1-4,共4页
Guizhou Agricultural Sciences
基金
贵州省重大专项"火龙果优异种质资源创新及工厂化育苗技术研究"(20126006-1)
国家自然科学基金"贵州喀斯特山地火龙果高抗旱种质应答干旱胁迫的分子基础"(31060256)
"逆境胁迫激发火龙果高频体细胞遗传变异的分子机理"(31260464)
贵州大学研究生创新基金"火龙果抗旱相关基因的克隆及表达分析"(研农2013003)
贵州大学引进人才科研项目"火龙果抗旱相关基因的表达分析及全长序列的克隆"(2012018)
