摘要
目的探讨胶体金免疫层析试验检测法（gold immunochromatography assay，GICA）在检测急诊病人乙型肝炎病毒表面抗原、丙型肝炎病毒抗体、人类免疫缺陷病毒抗体中的临床应用价值。方法随机选择2010年2月至2013年2月在四川省双流县第一人民医院就诊患者1080人作为研究对象，采集患者血清标本进行GICA与酶联免疫吸附测定（enzyme—linked immuno sorbentassay，ELISA）。以ELISA法检测结果为金标准，计算GICA检测乙型肝炎病毒表面抗原、丙型肝炎病毒抗体、人类免疫缺陷病毒抗体的灵敏度、特异度、漏诊率和准确性。结果用GICA检测乙型肝炎病毒表面抗原的灵敏度为91．3％（21／23），特异度为99．6％（1053／1057），漏诊率为8．7％（2／23），准确性为99．4％（1074／1080）；应用GICA检测抗丙型肝炎病毒抗体的灵敏度为57．1％（4／7），特异度为99．8％（1071／1073），漏诊率为42．9％（3／7），准确性为99．5％（1075／1080）。应用GICA检测人类免疫缺陷病毒抗体的灵敏度、特异度和准确性为100．0％。结论GICA可用于乙型肝炎病毒表面抗原和人类免疫缺陷病毒抗体的筛检，但对丙型肝炎病毒抗体的检测漏诊率高，不适宜用于丙型肝炎病毒抗体的筛检。
Objective To access the value of GICA in testing HBsAg, HCV-Ab and HIV-Ab in the emergency cases. Methods A total of 1080 serum specimens were collected from February 2010 to February 2013 in Shuangliu First People~ Hospital. ELISA and GICA were used to test. Taken ELISA as the gold standard, sensitivity, specificity, rate of fail to diagnosis and accuracy were calculated by using GICA. Re- suits The sensitivity, specificity, rate of fail to diagnosis and accuracy were 91.3%, 99. 6%, 8.7% and 99.4% respectively in testing HBsAg using GICA. In terms of HCV-Ab testing, the sensitivity, specificity, rate of fail to diagnosis and accuracy were 57. 1% , 99. 8%, 42. 9% and 99.5% respectively. The indexs of sensitivity, specificity and accuracy were 100% in testing HIV-Ab. Conclusions GICA is acceptable in screening HBsAg and HIV-Ab, but not suitable for screening HIV-Ab.
出处
《国际病毒学杂志》
2013年第4期189-191,共3页
International Journal of Virology
