摘要
[目的]通过研究Her-2免疫组化检测结果为不确定(2+)的乳腺癌患者的临床病理特征,寻找预测Her-2基因状态的方法。[方法]纳入51例Her-2免疫组化检测结果为2+的乳腺癌患者,采用荧光原位杂交法(FISH)确定其Her-2基因状态,比较Her-2基因扩增组与未扩增组之间的临床病理特征,通过组间差异寻找预测Her-2基因状态的指标。[结果]51例患者中,FISH检测Her-2基因未扩增者25例,扩增者26例。Her-2基因未扩增组与扩增组间患者的年龄、肿瘤大小、淋巴结状态、TNM分期、PR状态差异无统计学意义,ER状态差异有统计学意义。ER阳性率与Her-2基因扩增呈负相关。ER阳性细胞比>75%的患者主要(83.3%,15/18)存在于Her-2基因未扩增组中,ER阳性细胞比≤75%的患者主要(69.7%,23/33)存在于Her-2基因扩增组中。[结论]对Her-2免疫组化检测结果为2+的乳腺癌患者中,ER阳性细胞比率可粗略预测Her-2基因的状态。
[Purpose i] To investigate clinicopathnlogic features of breast cancer patients with imnmnohistochemisl,7 （IHC） Her-2 uncertain （2＋）,and to explore the method of predicting the status of Her-2 gene. [Methods] A total of 51 patients with hreast cancer whose Her-2 score was 2＋ were included. Fluorescence in situ hybridization （FISH） was applied to determine the status of Her-2 gene. The clinicopathological characteristics between Her-2 gene amplification group and Her-2 gene non-amplification group were compared and to search the differences be- tween the 2 gronps for predicting Her-2 gene status.[Results] FISH detection showed that of all the 51 patients,25 patients had no Her-2 gene amplification,while 26 patients had. As for age, tumor size,lymph node status,TNM stage and PR status,there was no statistical differenee be- tween the two groups,while statistical difference existed in ER status. ER positive was nega- tively correlated with Her-2 gene amplification. The patients whose ER positive cells proportion was 〉75% were mainly （83.3%. 15/18） from Her-2 gene non-amplification group.while those ER positive cells proportion ≤ 75% was mainly （69.7%,23/33） from Her-2 gene amplification group. [Conclusion ] For hreast cancer patient whose Her-2 score 2＋,ER positive cell propor- tion might roughly be used to predict the status of Her-2 gene.
出处
《肿瘤学杂志》
CAS
2013年第9期675-679,共5页
Journal of Chinese Oncology
基金
嘉兴市科技计划项目(2010AY1031)
