摘要
目的 研究依托咪酯对大鼠脑ｃＡＭＰ含量及腺苷酸环化酶（ＡＣ） 活性的影响。方法 ３０ 只ＳＤ 大鼠，随机分为３ 组，即对照组、依托咪酯１ 组和依托咪酯２ 组。对照组等容腹腔注射生理盐水５ ｍｌ／ｋｇ，依托咪酯１ 和２组分别腹腔注射依托咪酯６ ｍｇ／ｋｇ 和１２ ｍｇ／ｋｇ。前２ 组腹腔注射３ ｍｉｎ 后断头，后一组翻正反射消失后立即断头。分别采用蛋白竞争结合法及以非标记ＡＴＰ为底物的方法测ＳＤ大鼠各脑区（ 皮质、海马、脑干）的ｃＡＭＰ含量与ＡＣ活性变化。结果 依托咪酯１ 组大鼠行为变迟钝，依托咪酯２ 组大鼠翻正反射消失、皮层内ｃＡＭＰ含量升高且与对照组相比以及两剂量组间皆存在统计学差异（ Ｐ＜ ０．０５）。两组海马ｃＡＭＰ含量均高于对照组（ Ｐ＜０ ．０５）。依托咪酯对各脑区ＡＣ活性变化无明显影响（ Ｐ ＞ ０．０５）。结论 依托咪酯的全身麻醉作用与其升高皮质、海马ｃＡＭＰ的含量有关。
Objective To investigate the effects of etomidate on cAMP level and adenylate cyclase (AC) activity in rat brain. Methods 30 SD rats were divided randomly into 3 groups.The animals were intraperitoneally administered equal volumes of normal saline 5 ml·kg -1 (control group),etomidate 6 mg·kg -1 (etomidate 1 group)、 and etomidate 12 mg·kg -1 (etomidate 2 group), respectively. The rats in etomidate 2 group were decapitated immediately after the disappeareance of righting reflex, while those in control group and etomidate 1 group were decapitated 3 minutes after the injection of normal saline or etomidate.The rapidly removed brain stem, hippocampus and cerebral cortex, were homogenized and centrifuged. The cAMP level was assayed by protein competition binding analysis and the AC activity was analysed by taking non-radiolabelled ATP as the substrate. Results While righting reflex was lost in etomidate 2 group, the rats' be havior looked sluggish in etomidate 1 group. The cortical cAMP level in etomidate 2 group was increased, higher than in control and etomidate 1 group (P<0.05). The level of hippocamp al cAMP was increased in both etomidate groups (P<0.05).However,AC activities in the 2 etomidate groups were not affected (P>0.05). Conclusion The increment of cAMP level in hippocampus and cerebral cortex may be related to the anesthestic effect of etomidate.
出处
《徐州医学院学报》
CAS
2000年第1期1-3,共3页
Acta Academiae Medicinae Xuzhou
基金
江苏省教委自然科学基金!资助课题(No.96093)
