摘要
目的 寻找检测外周血中肝癌细胞的灵敏方法 ,以便早期发现肝癌患者的血行微转移灶。方法 采用逆转录和巢式多聚酶链式反应及Southern杂交技术 ,将AFPmRNA作为肝癌细胞的特异标志 ,特异扩增检测肝癌患者外周血中微量转移的肝癌细胞。结果 30例正常人和 2 1例肝转移癌均阴性 ,2 4例慢性肝病中有 1例阳性 ;83例肝癌患者有 5 1例阳性 ,阳性率 6 1.5 %。结论 用此方法可以较目前其它检查方法更早发现肝癌患者体内的血行微转移灶。
Objective To detect hematogenous spread of hepatocellular carcinoma earlier.Methods We used reverse transcript,nested PCR and southern hybridization with α fetoprotein mRNA which can be symbolized in hepatocellular carcinoma(HCC).Results Blood samples of thirty healthy controls and 21 other tumors metastasized to liver was negative.It was positive in 1 of 24(4.2%) benign liver diseases. 51 of 83HCC(61.5%) was positive.Conclusion Our test can find micromerastatic tumor in the patients with HCC by hematogenous spreading more earlier than examinations.
出处
《哈尔滨医科大学学报》
CAS
2000年第5期367-370,共4页
Journal of Harbin Medical University
关键词
肝细胞癌
肿瘤转移
聚合酶链反应
SOUTHERN杂交
messenger ribonucleic acid
α-fetoprotein
reverse transcript
nested polymerase chain reaction
hepatocellular carcinoma
