摘要
目的探讨川芎嗪对PD小鼠多巴胺神经元的保护作用。方法 30只健康雄性C57BL/6小鼠,随机分为正常对照组、模型组和治疗组。模型组和治疗组给予腹腔注射百草枯和代森锰建立PD模型,建模成功后,治疗组给予川芎嗪腹腔注射2 w,正常对照组给予同剂量生理盐水。观察3组小鼠行为学的改变、并用免疫组织化学、Western印迹法检测中脑多巴胺神经元数量、形态参数及Bcl-2的表达。结果模型组与治疗组比较,爬杆时间明显延长,中脑多巴胺神经元数量显著减少、Bcl-2的表达显著增高。结论川芎嗪治疗可上调Bcl-2的表达,增加黑质TH阳性神经元数量,有明显的神经保护作用。
Objective To investigate the effect of Tetramethy Lpyrazine on tyrosine hydroxylase（TH） in midbrain of Parkinson dis-ease（PD） mice model induced by paraquat and maneb. Methods Male C57BL/6 mices were randomly divided into PQ ＋ MN treated, Tet-ramethy Lpyrazine treated and control groups. Behavioral, immunohistochmical and western blot were used to detect the motor coordination and the expressions of TH, bcl-2 in midbrain. Results Administration of Tetramethy Lpyrazine could significantly attenuate the impairment of behavioral performance, increase the expression of TH and bcl-2 in midbrain. Conclusions Tetramethy Lpyrazine has a beneficial influ- ence on parkinsonism by increase bcl-2 in midbrain.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2013年第17期4173-4175,共3页
Chinese Journal of Gerontology
基金
贵州省科技厅联合基金项目〔黔科合LG字(2011)023号〕
贵州省社会发展科技攻关计划项目〔黔科合SY字(2008)3046号〕
