摘要
目的 :探讨新的人凋亡相关基因产物TFAR19蛋白对羟基喜树碱诱导人 772 1肝癌细胞凋亡的增敏作用。方法 :将不同浓度的rhTFAR19蛋白单独或与羟基喜树碱 (hydroxycamptothecin ,HCPT)同时加入处于指数生长期的 772 1肝癌细胞中共同培养 ,通过透射电镜、DNA片段化分析、PI及AnnexinV标记进行流式细胞仪分析 ,观察细胞的形态学和细胞凋亡的变化。结果 :不同质量浓度的rhTFAR19蛋白单独作用于 772 1肝癌细胞未见明显凋亡 ,但同时加 5mg·L-1的羟基喜树碱后 ,各质量浓度组均观察到细胞凋亡 ,并且显示了明确的量效关系 :5mg·L-1的HCPT加 5mg·L-1rhTFAR19蛋白的细胞凋亡率为 2 9.5 8% ,而加 2 0mg·L-1rhTFAR19蛋白的细胞凋亡率为6 3 .77%。结论 :rhTFAR19蛋白对HCPT诱导的细胞凋亡有明显的剂量依赖性促进作用 ,临床用HCPT行局部化疗时 ,若加rhTFAR19蛋白 ,可能会在不增加化疗药物毒性的情况下 。
Objective: To study the effect of rhTFAR19 protein on apoptosis of 7721 liver cancer cells induced by hydroxy camptothecin (HCPT). Methods: Cell morphology was observed by electronmicroscopy and analyzed by DNA fragmentation; PI and Annexin V labeled FACS assay were applied to study the apoptosis of 7721 liver cancer cells. Results : Differentmass mass concentrations of rhTFAR19 protein do not significantly affect apoptosis of 7721 liver cancer cells; but rhTFAR19 protein increased obviously the rate of apoptosis of 7721 liver cancer cells induced by 5 mg·L -1 HCPT, the rate of apoptosis was 29.58% when 5 mg·L -1 rhTFAR19 protein was added to 5 mg·L -1 HCPT; when cells were treated with 20 mg·L -1 rhTFAR19 protein plus 5 mg·L -1 HCPT, apoptosis reached 63.77%; especially early and middle phase apoptosis, with a dose dependent pattern. Conclusion: rhTFAR19 protein accelerate apoptosis of 7721 liver cancer cells induced by HCPT, so if the HCPT and rhTFAR19 protein are used to treat cancer patients, they may increase the effectiveness and lower the toxicities.
出处
《北京医科大学学报》
CSCD
2000年第5期408-410,共3页
Journal of Peking University(Health Sciences)
基金
国家自然科学基金!(39870 42 7)
