摘要
目的探讨CD4+T淋巴细胞异常表达程序性细胞死亡因子4(PDCD4)在不稳定型心绞痛患者经皮冠状动脉介入治疗(PCI)围术期的作用及意义。方法入选住院并行择期PCI的不稳定型心绞痛患者33例,同时选取29例只行冠状动脉造影检查而不行PCI的不稳定型心绞痛患者为对照组。分别于冠状动脉造影前或PCI术前、冠状动脉造影后或PCI术后18 h^24 h抽取新鲜外周血,免疫磁珠法分选出CD4+T淋巴细胞,荧光定量PCR检测PDCD4 mRNA表达,免疫印迹法检测PDCD4蛋白表达,酶联免疫吸附法检测血清肿瘤坏死因子α(TNF-α)浓度。结果与对照组相比,PCI组术后PDCD4 mRNA和蛋白表达明显升高(P<0.05)。PCI组术后血清TNF-α浓度较术前进一步升高(16.11±1.45 ng/L比7.60±0.75 ng/L;P<0.05),对照组无明显变化(P>0.05)。结论不稳定型心绞痛患者PCI术后CD4+T淋巴细胞PDCD4表达上调,从而增加PCI术后心肌的炎症反应。
Aim To investigate the effect and significance of programmed cell death 4 （PDCIM） abnormal ex- pression of CD4 ＋ T lymphoeytes in patients with unstable angina pectoris during perioperative period of percutaneous coro- nary intervention （PC1）. Methods A total of 62 unstable angina patients were enrolled. Of the 62 patients, 33 pa- tients undergoing PCI were involved as PCI group, the other 29 patients without PCI were set as control group. Peripheral blood was extracted before and after coronary angiography or PCI. Circulating CD4 ＋ T cells were obtained by magnetic cell sorting system （MACS） at baseline and 18 h ~ 24 h post-PCI. Real-time fluorescent quantitative polymerase chain reaction （RFQ-PCR）, Western blot analysis, enzyme-linked immunosorbent assay （ELISA） were used to evaluate the lev- els of PDCD4 mRNA in CD4 ＋ T lymphocyte, the expression of PDCD4 protein and also the serum level of tumor necrosis factor-or （TNF-ot）. Results Compared to the control group, the post-operative expression of PDCD4 mRNA and pro- tein dramatically increased in the PCI group （ P 〈 0. 05 ）. Compared to the pre-operation, the serum level of TNF-ct was increased in 18 h ~ 24 h post-operation in the PCI group （ 16.11± 1.45 ng/L vs 7.60± 0.75 ng/L; P 〈 0.05 ）. As to the level of TNF-α in the control group, no significant change was found （P 〉 0.05 ）. Conclusions The expression of PDCD4 was increased in patients with unstable angina pectoris undergoing percutaneous coronary intervention, which con- tributed to the intensive inflammation response in the myocardial level after PCI.
出处
《中国动脉硬化杂志》
CAS
CSCD
北大核心
2013年第8期727-731,共5页
Chinese Journal of Arteriosclerosis
基金
国家自然科学基金(81160046)资助
