摘要
目的:研究流感病毒感染对慢性阻塞性肺疾病急性加重(AECOPD)氧化应激和炎性反应的影响。方法:选取200例可疑病毒感染的AECOPD门诊患者,用RT-PCR方法检测患者痰液中流感病毒A。流感病毒感染患者为流感组,稳定期COPD患者为对照组。比较两组患者痰液丙二醛(MDA)、超氧化物岐化酶(SOD)水平,血浆IL-6和IL-8水平。分析流感病毒表达与氧化应激指标和炎症指标的相关性。结果:流感病毒感染患者25例。流感组痰MDA水平(6.64±1.22)nmol/ml,高于对照组,而SOD水平(58.47±6.79)U/ml,低于对照组,组间差异有统计学意义(P均<0.05)。流感组血浆IL-6和IL-8水平分别为(67.89±15.8)pg/ml和(5.81±1.09)ng/ml,均高于对照组(P<0.05)。流感组内,流感病毒表达强度与痰MDA水平相关(r=0.557,P﹤0.05),而痰MDA与血浆IL-8水平正相关(r=0.463,P<0.05)。结论:流感病毒感染引起的慢性阻塞性肺疾病急性加重患者气道氧化应激状态增强和全身炎症增强。
Objective To investigate oxidative stress and system inflammation of acute exacerbation of chronic obstructive ptdmonary disease induced by influenza virus. Method Two hundreds successive out - patients with AECOPD were enrolled to detect the influenza vi- ruses A （FluA） in sputum by RT- PCR. The AECOPD with positive Flu A in sputum were divided into Group Flu, and 20 stable COPD patients were selected as control. The content of MDA and activity of SOD in sputum, as well as the IL - 6 and IL - 8 in plasma were deter- mined with different assay kits. Results A higher level.of sputum MDA and lower activity of sputum SOD were detected in group FLu than control group（ P 〈 0. 05 ）. The plasma IL -6 and IL -8 were increased in group FLu than control group（ P 〈 0. 05 ）. There are significant correlation between sputum Flu and MDA （ r = 0. 557, P 〈 0. 05 ）, as well as sputum MDA and plasma IL- 8 （ r = 0. 463, P 〈 0. 05 ） . Conclusion There was remarkable deterioration in system inflammation in patients with acute exacerbation of chronic obstructive pulmona- ry disease induced by influenza virus, aeeompanylng with the serious imbalance of oxidative stress.
出处
《吉林医学》
CAS
2013年第25期5093-5095,共3页
Jilin Medical Journal
基金
山东省医药卫生科技发展计划项目[项目编号:2009H2028]
青岛市公共领域科技支撑计划项目[项目编号:10-3-3-4-3-nsh]
关键词
慢性阻塞性肺病
流感病毒
氧化应激
炎性反应
Chronic obstructive pulmonary dise^se
Lufluenza virus
Oxidative stress
Inflammation
