摘要
目的 :探讨 PCR-微孔板杂交法检测矽肺合并结核杆菌感染的可靠性和实用性。方法 :用 PCR-微孔板杂交检测、痰培养和涂片抗酸染色 3种方法对 10 5例矽肺患者的痰标本进行结核杆菌检测 ,同时与患者 X线胸片进行比较分析。。结果 :10 5例矽肺患者中 ,14例经 X线胸片检查发现合并结核 (占 13.3% ) ,PCR-微孔板杂交、痰培养和痰涂片抗酸染色 3种方法的结核杆菌阳性检出率分别为 2 6 .7%、14.3%和 4.8%。在 X线胸片未发现合并结核的矽肺患者中 ,通过PCR-微孔板杂交法检出 16例结核 ,痰培养阴性的 13例患者经 PCR-微孔板杂交法检出结核杆菌。结论 :对于矽肺合并结核感染的诊断 ,PCR-微孔板杂交技术是灵敏、可靠的检测方法 ,尤其适用于结核早期感染的诊断。
Objective In order to inquire the reliability and practicality of PCR-ELISA in detection of Mycobacterium tuberculosis infection relative to silicosis.Methods Sputum of 105 silicosis patients were detected with 3 methods of PCR-ELISA,sputum culture and antiacid staining of smear,which were compared with the X-ray chest radiographs of patients.Results 14 cases of pulmonary tuberculosis relative to silicosis were found by chest roentgenogram(13.3%).The positive detection rates of Mycobacterium tuberculosis by PCR-ELISA,sputum culture and antiacid staining of smear were 26.7%,14.3% and 4.8% respectively.16 cases of tuberculosis were found by PCR-ELISA among the negatives by chest roentgenogram,and 13 cases were found among the negatives by sputum culture.Conclusion PRC-ELISA is a sensitive and reliable detective method in diagnosis of silicotuberculosis,especially in early stage. [WT5”HZ]
出处
《职业卫生与病伤》
2000年第3期137-138,共2页
Occupational Health and Damage
关键词
PCR
微孔板杂交
矽肺
结核病
诊断
WT5”BZ]PCR-microwell plate hybridization Enzyme-linked immunosorbent assay(ELISA) Silicosis Tuberculosis Diagnosis
