摘要
[目的]观察鹿瓜多肽注射液对去势模鼠股骨生物力学的影响。[方法]将4月龄清洁级雌性SD大鼠40只随机分为假手术组(SHAM组),去势组(OVX组),去势+低剂量鹿瓜多肽组(OVX+CCP1组),去势+高剂量鹿瓜多肽组(OVX+CCP2组),每组10只。按照分组造模成功后,第13W起开始给药,分别为SHAM组:以0.9%NaCl肌注;OVX组:以0.9%NaCl肌注;OVX+CCP1组:以2mg.mL-1鹿瓜多肽注射液0.4mL/(kg.d)肌注;OVX+CCP2组:以2mg.mL-1鹿瓜多肽注射液0.8mL/(kg.d)肌注。用药频率均为1次/d,连续用药12W后断头法处死所有大鼠,采集右股骨测量骨生物力学数据。[结果]用药12W后,与SHAM组相比,OVX组生物力学性能明显下降(P<0.01);OVX+CCP1组,OVX+CCP2组在股骨的弹性载荷、最大载荷等参数与OVX组比较改善显著(P<0.05);但不及SHAM组水平,有显著性差异(P<0.05);OVX+CCP2组与OVX+CCP1差异具有显著性(P<0.05)。[结论]鹿瓜多肽注射液能够显著改善去卵巢后大鼠的骨生物力学性能,因此推论对雌激素水平低下的骨结构力学性能应具有维持和改善作用;鹿瓜多肽在高低两种不同剂量下对改善骨生物力学性能方面均表现出差异性,显示剂量依赖趋势,提示鹿瓜多肽注射液可能具有剂量依赖效应。
[Objective]To study whether Cervus and Cucumis Polypeptide（CCP,Lugua polypeptide） can treat osteoporosis or not. [Methods]Forty 4- month-old female SD rats were randomized into four groups（10 in each group） , including SHAM,0VX, OVX＋CCP1 and OVX＋CCP2 groups. Male rats in OVX groups take the ovariectomized surgery. Since the 13th week, rats in groups A and B got 0.9% physiological saline injection once in a day, group C was treated with CCP 0.4mL/（kg-d） injection and group D treated with CCP0.8mL/（kg＇d） injection once one day. Twelve weeks later, all rats were sacrificed for obtaining specimen. Took right femurs, measured the bone biomechanical properties. [Results ]After 12 weeks since injection, the biomechanical property of right femurs in group OVX was significantly lower than that of the SHAM group（P〈0.01）; parameters such as maximum load and elastic load in group OVX＋CCP1 and OVX＋CCP2 were higher than that of group OVX（P〈0.05）, but lower than that of group SHAM（P〈0.05）. The distinction of the above parameters was significant（P〈0.05） between group OVX＋CCP1 and OVX＋CCP2. [Conclusion]l.CCP can significantly im- prove the bone internal biomechanical properties in ovariectomized（OVX） rats, so it has function to treat osteoporosis caused by drop in estrogen level. There are differences of bone biomechanical properties aspects when treated with different dosage of CCP, which shows the tendency of dose-dependent effect. So we can conclude that CCP may have dose-dependent effect.
出处
《浙江中医药大学学报》
CAS
2013年第8期1015-1017,1022,共4页
Journal of Zhejiang Chinese Medical University
基金
浙江省中医药科技计划项目基金(2009CA009)~~
关键词
鹿瓜多肽
骨生物力学
剂量依赖效应
雌激素水平
骨质疏松症
cervus and cucumis polypepfide（CCP）
biomechanical properties
dose-dependent effect
estrogen level
osteoporosis
