摘要
目的建立测定复方雷尼替丁胶囊中盐酸雷尼替丁含量的方法。方法色谱柱:Kromasil C18(5μm,4.6mm×250 mm);柱温:35℃;流动相:0.6%磷酸溶液(用50%氢氧化钠溶液调节pH值至7.10±0.05)-乙腈(83:17);检测波长:230 nm;流速:1.0 mL min-1,进样量:10μL。结果盐酸雷尼替丁在0.010 85~0.54241 mg mL-1线性良好(r2=0.999 7),最小检出量为1.182 63 ng,平均回收率为99.8%(n=6),RSD为1.0%。结论建立的方法简便、灵敏、专属性好、结果准确,适用于复方雷尼替丁胶囊中盐酸雷尼替丁含量的测定。
Objective To establish an HPLC method to determine the content of ranitidine hydrochloride in compound ranitidine capsules.Methods The column was Kromasil C18(5 μm,4.6 mm×250 mm).The mixture of 0.6% Phosphoric acid solution(adjusting pH 7.10±0.05 with 50% sodium hydroxide solution)-acetonitrile(83 :17) was used as the mobile phase.The column temperature was 35 ℃,the flow rate was 1.0 mL min-1,the sample volume was 10 μL,and the detection wavelength was 230 nm.Results The linear range of ranitidine was 0.010 85-0.542 41 mg mL-1(r^2= 0.999 7).The limit of quantification was 1.182 63 ng.The average recovery was 99.8%(n = 6),RSD = 1.0%.Conclusion The method is simple,sensitive,selective and accurate,which can be used for the content determination of ranitidine hydrochloride in compound ranitidine capsules.
出处
《中南药学》
CAS
2013年第8期613-616,共4页
Central South Pharmacy