摘要
为印证角质酶在灰葡萄孢(Botrytis cinerea)穿透寄主植物组织过程中所起的重要作用,利用紫外诱变技术,在角质酶选择性培养基上筛选,获得了4个角质酶活性有变化的灰葡萄孢突变体菌株,与野生型菌株H2相比,突变体H2-M1和H2-M2表现出角质酶活性增强,而突变体H2-M3和H2-M4表现出角质酶活性下降。培养基上10次继代培养突变体菌株时未发现其产角质酶性状发生变化。无创伤接种番茄和草莓果实的试验结果表明,相比于野生型菌株,突变体H2-M1和H2-M2表现出致病力增强,而H2-M3和H2-M4的致病力弱于野生型菌株。但在有伤口果实上接种后,酶缺陷突变体H2-M3和H2-M4的致病力与野生型菌株相同。研究发现这4个突变体菌株产生其他致病因子(多聚半乳糖醛酸酶、果胶甲基多聚半乳糖醛酸酶、纤维素酶及毒素)的能力未受到紫外诱变的影响。研究结果表明,B.cinerea分泌的角质酶可能在其直接侵入寄主表皮的过程中起重要作用,但紫外诱变引起哪些基因位点突变,从而导致角质酶活性变化的原因则有待于进一步分析。
Cutinase of Botrytis cinerea has been proposed to play an important role in penetration of host tissues. In order to clarify the role of cutinase, by using ultraviolet mutagenesis method four mutants with altered cutinase activity were screened from cutinase-selective medium, two （H2-M1 and H2-M2） with increased and the other two （H2-M3 and H2-M4） with deficient cutinase activity compared to the wild-type isolate H2. Subcultivation of the mutants on medium for 10 times revealed the stability of effected cutinase activity of each mutant. Stronger virulence of H2-M1 and H2-M2, but reduction of virulence of H2-M3 and H2-M4 were observed on unwounded tomato and strawberry fruits compared to H2. However, H2-M3 and H2-M4 demonstrated full virulence on wounded hosts. These mutants retained full production abilities of other pathogenicity factors （polygalacturonase, pectin methylgalacturonase, cellulase and toxins）. Our data in- dicated that cutinase secreted by 13. cinerea may be involved in direct penetration of host cuticle. However, whether some gene sites were affected by ultraviolet light and therefore responsible for cutinase activity variation is yet to be investiga- ted.
出处
《扬州大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2013年第2期75-81,91,共8页
Journal of Yangzhou University：Agricultural and Life Science Edition
基金
国家自然科学基金资助项目(31101395)
江苏省自然科学基金资助项目(BK2011443)
浙江省宁波市重大科技项目(2009C10004)
关键词
灰葡萄孢
角质酶
突变
致病性
Botrytis cinerea
cutinase
mutation
pathogenicity
