摘要
研究了正常生长、低温胁迫和高氨氮胁迫下10个斑节对虾家系生长特性及超氧化物歧化酶(Superoxide Dismutase,SOD)、酸性磷酸酶(Acid Phosphatase,ACP)、酚氧化酶(Phenoloxidase,PO)3种免疫酶活性的变化。生长试验结果表明,10个家系特定生长率(SGR)呈现明显差异,家系077与家系017和661的ACP活性存在极显著差异,家系4612与家系017的SOD活性存在极显著性差异,而其他家系的免疫酶活性无显著差异。斑节对虾在48h低温胁迫下,ACP和SOD活性先升高后降低,PO活性则不断降低,而对照组的3种酶活性呈无规律轻微变化。斑节对虾在48h高氨氮胁迫下,ACP活性不断升高,SOD在0—8h活性上升,8—48h活性快速降低,PO活性不断下降,而对照组的3种酶活性呈无规律轻微变化;一些家系间的3种免疫酶活性存在显著性差异,其中家系4612的3种酶活性均高于其他家系。3个试验中,家系的特定生长率与免疫酶活性均呈负相关。
The response of Superoxide Dismutase （SOD）, Acid Phosphatase （ACP）, and Phenoloxidase （PO） in Penaeus monodonfamilies to normal growth, low temperature stress and high ammonia stress was studied under laboratory condition. Growth in terms of specific growth rate （SGR） was significant difference among the selected families （p〈0.05）. The activities of ACP were significant difference among family 077, 017 and 661. The activities of SOD were significant difference between family 4612 and family 017. When the rearing temperature decreased from 27 ℃ to 20 ℃, the activities of ACP and SOD first increased and then decreased, the activities of PO reduced prolonged according to the low temperature stress, and the minor changes of control trial were not regular. Under the high ammonia stress, the activities of ACP increased prolonged, the activities of SOD increased in 0-8 h, then rapidly decreased in 8-48 h, the activities of PO reduced in 0-48 h, and the minor changes of control trial were not regular. The activities of immune enzymes of some families were significant differences, and three immune enzymes activities of family 4612 higher than that of other families under the high ammonia stress. A negative correlation was found between the specific growth rate and the activities of ACP, SOD and PO of ten families in three trials.
出处
《热带海洋学报》
CAS
CSCD
北大核心
2013年第3期78-85,共8页
Journal of Tropical Oceanography
基金
国家高技术研究发展("863")计划项目(2012AA10A409-02)
广东省科技计划项目(2009B020308002)
广东省自然科学基金项目(10451030002005817)
广东省海洋渔业推广专项(A201101B03
A201201C01)
海南省重点科技计划项目(ZDXM20110016)
关键词
斑节对虾
家系
氨氮
低温
免疫酶
Penaeus monodon
family
low temperature
ammonia
immunity enzymes activity
