摘要
目的:观察益水生新饮对自发性高血压大鼠(SHR)肾脏组织的保护作用及可能的机制。方法:将12周龄雄性SHR大鼠随机分成6组,分别灌胃益水生新饮低、中、高3个剂量,阳性药为苯那普利,设模型对照组和空白对照组。给药8周后测血压并处死大鼠。检测大鼠尿微量白蛋白,血尿素氮和胱抑素水平;HE及Masson染色切片法观察各组大鼠肾脏病理改变;免疫组织化学方法检测肾组织TGF-β1,CTGF,FN的表达变化。结果:与空白对照组比较,模型组SHR大鼠血压明显升高;与模型对照组比较,益水生新饮低、中、高组和苯那普利组同期血压明显降低。与空白对照组比较,模型组尿微量白蛋白、血尿素氮及胱抑素水平升高;与模型对照组比较,益水生新饮中、高组和苯那普利组尿微量白蛋白、血尿素氮及胱抑素明显降低。病理检查提示,模型组比给药各组病变明显加重,出现肾小球肥大及肾小球基底膜非均质增厚并广泛空泡变性等病理改变。模型组大鼠肾组织TGF-β1,CTGF,FN表达明显上调(P<0.01),益水生新饮中、高剂量组及苯那普利组TGF-β1,CTGF,FN表达明显下调(P<0.05)。结论:益水生新饮能够减轻SHR大鼠早期肾脏肾小球硬化和肾间质纤维化的程度,起到延缓高血压肾损害的进展和肾脏保护的作用,其作用机制与TGF-β1,CTGF,FN信号通路有关。
This study was aimed to observe renoprotective effect and possible mechanism on Yi-Shui Sheng- Xin Yin in spontaneously hypertensive rats. Sixty 12-week male SHR rats were randomly divided into six groups, which were the Yi-Shui Sheng-Xin Yin low-dose group, middle-dose group, high-dose group, Benazepril group, model group and blank control group, and ten rats for each group. The SHR rats were sacrificed after eight weeks. The urine microalbumin, blood urea nitrogen and The HE and Masson staining method were used to observe changes of renal of transforming growth factor-β1 （TGF-β1）, connective tissue growth factor cystatin were tested in each rat. pathology. Changes of expression （CTGF）, FN were detected by immunohistochemistry. The results showed that compared with the blank control group, blood pressure in model group was associated with a significant rise after 8 weeks. Compared with the model group, blood pressure in the Yi-Shui Sheng-Xin Yin middle-dose group, high-dose group and Benazepril group significantly decreased. Compared with the blank control group, urine microalbumin, blood urea nitrogen and cystatin in model group were associated with a significant rise. Compared with the model group, urine microalbumin, blood urea nitrogen and cystatin in the Yi-Shui Sheng-Xin Yin middle-dose group, high-dose group and Benazepril group significantly decreased. Pathological examinations showed that pathological changes in model group were faster than all drug-groups, appeared pathological changes of glomerular hypertrophy, glomerular basement membrane thickening of heterogeneity and extensive vacuoles degeneration. Immunohistochemical staining showed that compared with the blank control group, expressions of TGF-β1, CTGF and FN of rat kidney tissue in model group were obviously up-regulated （P 〈 0.05）. Compared with the model group, expressions of TGF- β1, CTGF and FN in the Yi-Shui Sheng-Xin Yin, middle-dose group, high-dose group and Benazepril group were down-regulated （P 〈 0.05）. It wa
出处
《世界科学技术-中医药现代化》
北大核心
2013年第5期975-981,共7页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
广西省科技厅自然科学基金(2011GXNSFA018218):益水生新饮对高血压早期肾损害的防治作用及机制研究
负责人:徐宏
广西中医药管理局中医药科技专项(GZYZ1122):益水生新饮对高血压早期肾损害的防治作用及机制研究
负责人:徐宏
关键词
益水生新饮
高血压肾损害
转化生长因子-Β1
结缔组织生长因子
Yi-Shui Sheng-Xin Yin, hypertensive kidney lesion, transforming growth factor-β1 （TGF-β1）,connective tissue growth factor （CTGF）
