摘要
目的:探讨明胶-白芨胶/丹参多孔材料复合大鼠真皮成纤维细胞制成组织工程真皮。方法:制备明胶-白芨胶/丹参多孔材料及其浸提液,与L-929细胞共同培养,观察浸提液与L-929细胞相容性。提取大鼠真皮层中成纤维细胞,与材料复合构建组织工程真皮。复合组织采用苏木精-伊红染色及电镜下观察结合效果。结果:L-929细胞在浸提液中生长情况良好。大鼠真皮成纤维细胞与材料共同培养2周,苏木精-伊红染色及电镜观察,细胞能在材料上正常粘附与生长。结论:明胶-白芨胶/丹参多孔材料无细胞毒性。能与大鼠真皮成纤维细胞成功复合为组织工程真皮。
Objective ：To compose the tissue-engineering corium by gelatin-bletilla/Salvia-miltiorrhiza material and fibroblast of rat corium. Method：The gelatin-bletilla/ Salvia-miltiorrhiza material and the leaching liquor of material were prepared. The leaching liquor was cultured with L-929 cells. The tissue-engineering corium was constructed by gelatin-bletilla/ Salvi a-miltiorrhiza material combined with fibroblast extracted from rat corium. The effect of combination was observed by TEM and HE staining. Result ： Leaching liquor did not cause cytotoxicity to L-929 cells when they were co-cultured. Fibroblast had got normal growth and adhesion in the material after 2 weeks. Conclusion：The gelatin-bletilla/Salvia-miltiorrhiza as porous material has no cytotoxicity. The material can be composed with fibroblast of rat corium as a tissue-engineering corium.
出处
《中国中医骨伤科杂志》
CAS
2013年第9期4-6,共3页
Chinese Journal of Traditional Medical Traumatology & Orthopedics
基金
国家自然科学基金资助项目(81102736)
中央高校基本科研业务费专项基金科研项目(302274991)
关键词
明胶-白芨胶
丹参
多孔材料
组织工程真皮
Gelatin-bletilla
Salvia-miltiorrhiza
Porous material
Tissue-engineering corium
