摘要
细菌是土壤生物的重要类群，由于其形态简单，绝大多数无法人工培养，用传统分类法 极难进行土壤细菌遗传多样性分析。选择土壤细菌１６Ｓ核糖体ＲＮＡ基因内一段２６碱基变异 区为基因标签，连接成标签串测序，用标签类型、频率和多样性指数等在分子水平上描述土壤 细菌遗传多样性，并分析了土壤细菌遗传多样性与植被类型的相关性。土壤细菌遗传多样性 与土壤有机质、全氮含量等理化性质高度相关，不同植被土壤理化性质差异决定细菌遗传多样性分化。
Soil is an important epicenter of biodiversity. Human activity has threatened such biodiversity through vegetation shift. As the major type of microbes, bacteria have played key roles in material cycling. Since simple morphological characters and the failure of the major portion of bacteria in surviving artificial cultivation have hindered bacterial diversity from analyses using traditional taxonomic approaches, bacterial diversity and its correlation with vegetation is less understood. In order to detour cultivation, tag serial sequencing strategy was developed in thes study, in which a 26 base pair highly variable region has been chosen from 16S ribosomal RNA gene as valiant-representing tags, ligated into serials and sequenced. Molecular bacterial diversity in soils has been determined using tag types, tag frequencies and diversity index as parameters. The correlation of bacterial diversity with vegetation has been explored as well. Bacterial diversity and differentiation correlate highly with contents of organic matter and nitrogen of soils and further vegetation.
基金
湖北省自然科学基金
武汉市科学技术委员会晨光计划项目资助
关键词
土壤
细菌
遗传多样性
植被
soil
bacteria, genetic diversity
vegetation
