摘要
以苦豆子和披针叶黄华种子为实验材料,在恒温25℃下吸胀,每24h取出吸胀种子,10d后未吸胀的种子为硬实种子(H),硬实种子用硫酸处理后恒温吸胀24h,与非硬实种子进行发芽试验和各项活力指标测定。结果显示:(1)每日内吸胀的种子数量随时间推移以一定比列下降,苦豆子非硬实种子吸胀速率较缓慢,第10天后吸胀率降到1%,披针叶黄华非硬实种子第3天后下降到1%,第9、10天突然上升后又下降到1%。(2)两种豆类种子都显示出硬实种子发芽率、发芽指数、活力指数、脱氢酶、呼吸速率、超氧化物歧化酶(SOD)均高于非硬实种子,而电导率、浸出液可溶性糖和丙二醛(MDA)均低于非硬实种子,缓慢吸胀的硬实种子活力指标高于快速吸胀的硬实种子。研究表明,硬实种子活力高于非硬实种子,硬实种子吸胀过程中存在吸胀损伤。而在非硬实种子中,根据以上活力指标判断,晚吸胀的种子比早吸胀的种子活力高。
Using Sophora alopecuroides and Thermopsis lanceolata as materials,under 25 ℃,the imbibed seeds were taken out as a sample every 24 h.The non-imbibing seeds after 10 d were regarded as hard seed （H）.Imbibing seeds and hard seeds that imbibed for 24 h after treated with sulfuric acid were all subjected to germination test and several vigour tests.The results indicated that：（1）The numbers of the seeds newly imbibed in one day were gradually decreased along with time.The hard seeds of S.alopecuroides had a relative slow rate of imbibition,which was decreased below 1% after 10 d,while the imbibition rate of T.lanceolata was at the same level after 3 d,another decrease happened after a sudden increase in 9th to 10th day.（2）The germination rate,germination index,vigour index,the activity of dehydrogenase,respiration rate and SOD activity of hard seeds were significantly higher than those of non-hard seeds in both species,while the relative conductivity,the contents of soluble sugar and malondialdehyde of hard seeds were lower than those of non-hard seeds.Studies showed that the vigour of hard seeds were higher than that of imbibing seeds,and the vigour of imbibing seeds were increased gradually with the initial imbibing time postponed.
出处
《西北植物学报》
CAS
CSCD
北大核心
2013年第8期1665-1673,共9页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家中医药管理局中草药特色科研基金(201207002)
关键词
苦豆子
披针叶黄华
硬实种子
非硬实种子
种子活力
吸胀损伤
Sophora alopecuroides
Thermopsis lanceolata
hard seed
non-hard seed
seed vigour
soaking injury
