摘要
为了解广东省规模化猪场猪流感病毒(SIV)感染情况,本研究采用ELISA方法和血凝抑制试验(HI),对2011年～2012年采集的1 050份血清样品进行SIV血清学检测。两种检测方法结果显示1 050份血清样品中SIV抗体阳性率分别为50.4%(ELISA)和50.2%(HI)。其中珠三角地区和粤东地区的感染率高于粤西地区。SIV亚型调查结果显示该地区流行的SIV主要为H1和H3亚型,抗体阳性率分别为39.2%和18.2%(ELISA)。部分猪场存在H9亚型SIV抗体。部分猪群中同时存在H1和H3两种亚型SIV抗体,表明猪群中存在不同亚型SIV混合感染。本研究为广东省猪流感的预防提供参考依据。
To investigate swine influenza virus （SIV） infection on large-scaled pig farms in Guangdong province, a total of 1,050 swine serum samples, collected from 2011 to 2012, were detected for the antibody against SIV by ELISA and HI assay. The results showed that 52.0% （ELISA） or 50.2% （HI） serum samples tested was positive for SIV, and the positive rates in Pearl River Delta and Shantou area were higher than that in Western Guangdong. H1 and H3 subtypes SIV were common in the tested pig farms, of which the antibody positives were 39.2% and 18.2% by ELISA detections, respectively. While antibodies to H9 subtype SIV were only found in several pig farms. In addition, some pig sera were positive to both H1 and H3 subtypes, indicating the mixed infection existed. These data provided reference basis for the prevention of swine influenza.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2013年第9期711-714,共4页
Chinese Journal of Preventive Veterinary Medicine
基金
现代农业产业技术体系(CARS-36)
