摘要
以“大红袍”枇杷果实为试材 ,研究了SO2 处理对冷藏枇杷果实活性氧代谢和内源多胺含量变化的影响及与果实木质化败坏的关系。结果表明 ,SO2 可提高超氧物歧化酶 (SOD)和过氧化氢酶 (CAT)活性 ,抑制过氧化物酶 (POD)活性的上升 ,减少H2 O2 的积累。经SO2 处理的果实 ,精胺 (Spm)和亚精胺 (Spd)含量的变化很小 ,基本维持在贮前水平上 ,腐胺 (Put)的积累则受到极大抑制 ,贮藏 5周后的果实不出现木质化败坏症状。这些结果表明 ,SO2 可能通过维持活性氧代谢的平衡 ,抑制活性氧的积累 。
Changes in active oxygen and polyamine metabolism and their relation to flesh woodiness development in SO 2 treated cold stored loquat (Eriobotrya japonica Lindl. cv. Dahongpao) fruits were investigated. The results indicated that SO 2 promoted the activities of superoxide dismutase (SOD) and catalase (CAT) (Fig.1A, B), inhibited the increase in peroxidase (POD) activity (Fig.1C) and H 2O 2 level (Fig.2). In fruits treated with SO 2, little changes were detected in spermine (Spm) and spermidine [HJ6”:2](Spd) levels throughout the experimental period (Fig.3A, B), the accumulation of putrescine (Put) was greatly inhibited (Fig. 3C), and no symptoms of flesh woodiness was observed after 5 weeks. The experimental results suggest that SO 2 may maintain the balance between the formation and detoxification of active oxygen species and inhibit their accumulation, thereby preventing the development of flesh woodiness in cold stored loquat fruits.
基金
南京农业大学人才基金资助项目
关键词
枇杷果实
冷藏
SO2
活性氧
多胺
果肉木质化
loquat fruits, cold storage, SO_2, active oxygen, polyamines, flesh woodiness
