摘要
为探讨单倍体相合造血干细胞移植(hi-HSCT)术后早期肺部感染患者的免疫状态,监测25例患者在移植前及移植术后6个月内不同时间点CD4+T细胞功能,了解其在肺部感染组和非肺部感染组患者间的差异,为调整免疫抑制剂用量提供参考,以减少肺部感染的发生。采用ImmuKnow方法检测患者外周血CD4+T细胞的ATP含量,评估T细胞功能。结果表明:移植后早期肺部感染组患者移植前CD4+T细胞ATP含量较低,为(179.88±65.41)ng/ml,移植后1个月降至(172.69±118.81)ng/ml,其后逐渐增高,但移植后3个月仍未达正常水平,为(218.15±124.26)ng/ml,移植后6个月升至正常,为(313.42±116.29)ng/ml。无肺部感染组患者移植前CD4+T细胞ATP含量也偏低,为(210.44±94.71)ng/ml,移植后1个月降至(193.66±133.69)ng/ml,其后逐渐上升,移植后3个月升至(355.02±43.38)ng/ml,移植后6个月为(355.73±93.85)ng/ml。结论:移植前后CD4+T细胞ATP含量均可提示移植后感染并发症的发生,可作为临床经验性抗生素应用的参考指标。
This study was purposed to investigate the immune state of the patients suffered from pulmonary infection within 6 months after haploidentical hematopoietic stem cell transplantation（hi-HSCT）.Adenosine triphosphate（ATP） value in CD4^＋ T cells was measured by ImmuKnow method to assess the function of the lymphocytes in peripheral blood of 25 patients at 6 months after hi-HSCT.The results showed that the ATP level in CD4^＋ T cells of the patients suffered from pulmonary infection was（179.88±65.41） ng/ml before transplantion,（172.69±118.81） ng/ml at 1 month,（218.15±124.26） ng/ml at 3 months,（313.42±116.29） ng/ml at 6 months after transplantion.The ATP level in CD4^＋ T cells of the patients without pulmonary infection was（210.44±94.71） ng/ml before transplantion,and decreased to（193.66±133.69） ng/ml at 1 month and increased gradually to（355.02±43.38） ng/ml at 3 months,（355.73±93.85） ng/ml at 6 months after transplantion.It is concluded that the low ATP value in CD4^＋ T cells in patients prior and post hi-HSCT may suggest probability of occurrence for infections,ATP value in CD4^＋ T cells may be used as a reference indicator for clinical empirical use of antibiotics.
出处
《中国实验血液学杂志》
CAS
CSCD
北大核心
2013年第4期995-998,共4页
Journal of Experimental Hematology
基金
国家自然科学基金面上项目(编号30871018
30971068
81170522)
国家高新科技项目(863)(编号2011AA020101)
广东省开发区科技局项目(编号2009Q-P081)
关键词
单倍体相合造血干细胞移植
肺部感染
CD4+T细胞功能
haploidentical hematopoietic stem cell transplantation
pulmonary infection
CD4^＋ T cell function
