摘要
拟通过用不同浓度的蟛蜞菊毛状根的乙醇提取液浸泡三叶鬼针草(Bidens pilosa L.)等种子研究其化感作用,为今后开展通过南美蟛蜞菊离体培养毛状根来生产高效低毒的"生物农药"及其应用奠定实验和技术基础。用0.5g/mL、1.0g/mL的蟛蜞菊毛状根的乙醇提取液,分别浸泡三叶鬼针草(Bidens pilosa L.)等种子3、12、24h后,进行种子萌发实验。以清水浸泡后的种子萌发情况作为对照相比。提取液的浓度越高或浸泡时间越长,种子的萌发率越低,而且发现在0.5g/mL(鲜重)的低浓度提取液中浸泡12h以上,鬼针草、马唐和狗尾草已经不能正常萌发,在1.0g/mL(鲜重)的高浓度提取液中浸泡12h以上对种子的抑制作用更明显,鬼针草、马唐、稗草、狗尾草、蒲公英种子均不能萌发。而且发现无论是用0.5g/mL(鲜重)的低浓度提取液还是用1.0g/mL(鲜重)的高浓度提取液进行浸泡,浸泡时间在24h以上,抑制效果就更加明显。蟛蜞菊毛状根具有较强的化感作用。
In this test, in order to lay the experimental and technological foundation for the production and application of making bio-chemicals with in vitro cultured hairy roots derived from Wedelia trilobata, seeds of several plants （Bidens pilosa L.） were soaked with ethanol extracts from Wedelia trilobata （L.） A.S.Hitche hairy roots with different concentrations, and study the allelopathic effect. The seeds （Bidens pilosa L. etc.） were immersed individually in ethanol extracts from Wedelia trilobata hairy roots of two concentrations, 0.5 g/mL and 1.0 g/mL, for 3 hours, 12 hours and 24 hours to do germination experiments. And as a contrast, another set of seeds were soaked with water. The higher the concentration of the ethanol extract was, or the longer the seeds were soaked, the lower the seeds germination rate would be. And it＇ s found that having been immersed in the 0.5 g/mL （fresh weight） low concentration extract for over 12 hours, the seeds of Bidens pilosa L., Dig#aria ischaemum, D.sanguinalis and Setaria viridis （L.） Beauv. could not germinate properly; while the inhibition effect on the seeds was more obvious when the seeds were soaked in the extract of 1.0 g/mL （fresh weight） high concentration for over 12 hours-seed of Bidens pilosa L., Digitaria iscbaemum, D, sanguinalis, （L.） Beauv., Setaria viridis （L.） Beauv. and Herba taraxaci could not germinate under this condition. What＇ s more, the test showed that when the soaking time was over 24 hours, no matter the concentration was 0.5 g/mL or 1.0 g/mL, the inhibition effect of the ethanol extracts would be much stronger. Wedelia trilobata hairy roots have strong allelopathic effect.
出处
《中国农学通报》
CSCD
2013年第24期58-61,共4页
Chinese Agricultural Science Bulletin
关键词
南美蟛蜞菊
毛状根
乙醇提取液
化感作用
种子萌发
Wedelia trilobata （L.） A.S.Hitche
hairy root
ethanol extracts
allelopathic effect
seed germination
