摘要
基于家蚕(Bombyx mori)Ras2基因的cDNA序列设计引物,利用逆转录聚合酶链式反应(RT-PCR)克隆了野桑蚕(Bombyx mandarina)Ras2基因,得到的cDNA序列长631bp,含有一个603bp的完整开放阅读框,由2个外显子和1个内含子组成,编码一个由200个氨基酸残基组成的蛋白质.该蛋白质的等电点为6.33,分子量为22.866kD.其氨基酸序列与其他昆虫Ras2氨基酸序列的同源性较高,且具有它们Ras2基因的典型特征.组织表达谱表明,该基因mRNA在野桑蚕的表皮、马氏管、中肠、脂肪体、丝腺中均有不同程度的表达.
The complemental deoxyribonucleic acid（cDNA） of Bombyx mandarina Ras2 gene was cloned by reverse transcription-polymerase chain reaction（RT-PCR）,the primer was designed based on the cDNA sequence of Bombyx mori Ras2 gene.The results showed that the cDNA with 631 bp in length contained an open reading frame（ORF） of 603 bp which encoded 200 amino acid residues,contains 2 exons and 1 intron,and a predicted molecular weight of 22.866 kD and isoelectric point of 6.33.The deduced amino acid sequence had a high identity to reported sequence of Ras2 from other insect species and shared the typical structural features of Ras2 with other insects.The Ras2 mRNA was expressed differently in epidermis,malpighian tubule,fat body,midgut,and silk gland of B.mandarina by RT-PCR analysis.
出处
《河南大学学报（自然科学版）》
CAS
北大核心
2013年第4期431-434,共4页
Journal of Henan University:Natural Science
基金
河南省教育厅自然科学基金项目(2010A230016)
周口师范学院博士科研启动基金资助项目
周口师范学院校级重点学科建设经费资助
关键词
野桑蚕
Ras2基因克隆
序列分析
组织表达
Bombyx mandarina
Ras2 gene cloning
sequence analysis
tissue expression
