摘要
为了有效利用玉米自交系种质资源,促进玉米育种进程,采用凝胶电泳方法研究了32个不同耐盐碱性的玉米自交系过氧化物同工酶的谱带迁移率和聚类分析情况。结果表明:自交系品种过氧化物同工酶谱带位点及带级变化较大,谱带分布不同,且有强弱变化;32个自交系材料共出现258条酶带,大致可划分为3个区:A区(0~0.13),B区(0.26~0.43),C区(0.55~0.81);过氧化物同工酶的欧氏距离变化范围为0~3.000,平均为1.500;当阈值取20时,供试品种可聚为10类。
In order to use the germplasm resources of maize inbred lines effectively and promote the breeding process, band mobility and cluster analysis of peroxidase isozyme of 32 maize inbred lines with different salt tol- erance were studied by gel electrophoresis. The results showed that the band sites and band classes changed greatly,distribution of bands were different and strength change^There were 258 bands which could be divided into three zones： A zone（0-0.13） ,B zone（0.26-0.43）, C zone（0.55-0.81 ） ;the change scope of euclidean distance was 0 -3. 000,the average was 1. 500 ;when the threshold was 20, tested varieties could be clusteredinto 10 types.
出处
《黑龙江农业科学》
2013年第9期5-8,共4页
Heilongjiang Agricultural Sciences
