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电子束辐射对铜绿微囊藻蛋白质及抗氧化体系酶活性的影响 被引量:2

Effect of Irradiation of Electron Beam on Protein and Antioxidized Enzyme Activity of Microcystis aeruginosa
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摘要 铜绿微囊藻以其毒素和产生的嗅味威胁人类健康和安全,且因大量生长和难去除性给水处理带来困难。本文以电子加速器产生的电子束对铜绿微囊藻培养液(对数生长期)分别进行1、2、3、4和5kGy剂量的辐射,通过考察藻细胞蛋白质、抗氧化体系酶活性及光合速率的变化,研究辐射对铜绿微囊藻生命特征及机能产生的影响。结果表明:1kGy的辐射对藻细胞可溶性蛋白质及POD、SOD活性的影响较小;而2~5kGy的辐射明显降低了蛋白质含量,并破坏了藻细胞的抗氧化系统,光合速率也因辐射明显降低,使铜绿微囊藻短时间内失活。表明一定剂量的电子束辐射能有效控制藻细胞生长,并影响其重要生命特征。 Microcystis aeruginosa often threatens human health and safety for its micro- cystin and bad smell. Its large number and hardness of removal are difficulty for water treatment. In this study, electron beam generated by an accelerator was applied to irra- diate Microcystis aeruginosa by dose of 1, 2, 3, 4 and 5 kGy. The effect of irradiation on Microcystis aeruginosa characteristic and mechanism was studied by surveying the changing of protein, enzyme activity and photosynthesis rate. The data show that irradi- ation of 1 kGy has little effect on dissoluble protein, POD and SOD activity. Irradiation of 2-5 kGy can decrease protein content and destroy the antioxidant system, also the photosynthesis rate decreases obviously, which makes Microcystis aeruginosa lose activ- ity in short time. The result proves that a certain dose of electron beam irradiation can control algae growth and affect its life characteristic efficiently.
出处 《原子能科学技术》 EI CAS CSCD 北大核心 2013年第8期1307-1310,共4页 Atomic Energy Science and Technology
基金 国家自然科学基金资助项目(50809037) 上海市重点学科资助项目(S30109) 城市水资源与水环境国家重点实验室开放课题资助项目(ES201004)
关键词 辐射 蛋白质 抗氧化体系 酶活性 光合速率 irradiation protein antioxidant system enzyme activity photosynthesis rate
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