摘要
目的制备一种能够超声/荧光双模式成像的造影剂,通过动物实验观察其对淋巴管及淋巴结超声造影和近红外活体荧光成像的效果。方法组装嵌合淋巴结靶向配体(磷脂酰丝氨酸)和近红外荧光物质的双功能靶向造影微泡,行光学显微镜及粒径检测,观察其形态与大小。选取5只正常新西兰大白兔,于双侧足垫注射双功能造影剂,同时观察其淋巴结、淋巴管超声造影及近红外荧光表现。于同部位注射活性蓝染料,5min后处死动物后取淋巴结行病理检查。结果脂质微泡超声造影剂粒径分布均匀、形态规则,直径约3～5μm。超声造影剂经皮下注射后可很快显示流入的淋巴管及相应淋巴结,近红外荧光成像与超声造影图像有良好的一致性。解剖证实所有蓝染的淋巴结均可荧光成像。结论自制双功能超声造影剂经皮下注射对兔正常淋巴结的造影效果良好,且同步可行近红外荧光成像,有望成为一种淋巴结示踪的良好方法。
Objective To prepare a lymph node-targeted ultrasound/fluorescence bi-functional imaging contrast agents, and observe its effectiveness both on contrast-enhanced ultrasound （CEUS） and vivo near infra- red fluorescence （NIR） imaging through animal experiments. Methods The chimeric lymph node-targeted lig- and （phosphatidylserine） and near-infrared fluorescent substance were assembled to form bi-funetional contrast microbubbles. The morphology and size distribution were detected by optical microscope and Malvern potential tests. Five normal New Zealand white rabbits were subcutaneously injected with the prepared contrast agent in bi- lateral footpads, and the imaging effectiveness of lymph nodes and lymphatic vessel were observed by CEUS and NIR technique. Then blue dye was subcutaneously injected at the same site, and the rabbits were sacrificed for lymph nodes pathological examination. Results Lipid ultrasound microbubbles, with a mean size of 3-5 μm in diameter, appeared to be uniform in distribution and regular in configuration. The images of inflow lymphatic ves-sel and relevant lymph node were quickly showed up after the subcutaneous injection by CEUS, which was identi- cal to the result detected by NIR. Biopsy confirmed that all the blue-stained lymph nodes could be displayed by NIR. Conclusions The self-made bi-functional contrast agent has a good imaging ability in CEUS and NIR ima- ging. It may be a better agent as lymph node tracer.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
2013年第4期411-415,共5页
Acta Academiae Medicinae Sinicae
基金
国家自然科学基金(81171347)~~
关键词
超声造影
荧光成像
微泡
淋巴结
uhrasound contrast
fluorescence imaging
microbubbles
lymph node
