摘要
目的:检测伴(N+)和不伴(N-)颈部淋巴结转移的喉鳞状细胞癌(LSCC)组织中泛素的表达情况,探讨其与LSCC颈部淋巴结转移及其他病理参数的相关性。方法:通过免疫组织化学技术结合中国体视学图像分析系统检测泛素在19例LSCC(N+)与20例LSCC(N-)组织的表达水平并作t检验、方差分析、ROC曲线等统计学分析。结果:泛素在LSCC(N+)与LSCC(N-)中的表达差异有统计学意义(P<0.01);其表达水平与是否伴颈部淋巴结转移有关,与年龄、烟酒嗜好史、临床分期、原发部位等均无关。结论:泛素在LSCC(N+)中的表达明显上调,提示其可能是参与LSCC发生淋巴结转移的重要分子之一。
Objective： To investigate the ubiquitin expression in laryngeal squamous cell carcinoma （LSCC） whether along with local lymph node metastasis, and further study its correlation with local lymph node metastasis and other clinicopathological parameters in laryngeal squamous cell carcinoma. Method： We detected the different expression level of ubiquitin in paraffin specimens between 19 cases of LSCC associated with cervical lymph node metastasis LSCC（N＋） and 20 cases of LSCC not associated with cervical lymph node metastasis LSCC（N--） by immunohistochemical staining combined with stereology image analysis system. Statistics were analyzed by student test, variance analysis and ROC curve. Result： Ubiquitin expression in LSCC（N＋ ） was significantly higher than LSCC（N--） （P〈0.01）; their expression level was not correlated with age,history of tobacco, alcohol addiction , clinical stage and primary site, etc. Conclusion： Ubiquitin was significantly up-expressed in LSCC（N＋ ） than LSCC （N--）, which may imply that it is one of the important elements in mechanism of lymph node metastasis in LSCC.
出处
《临床耳鼻咽喉头颈外科杂志》
CAS
北大核心
2013年第17期944-947,共4页
Journal of Clinical Otorhinolaryngology Head And Neck Surgery
基金
国家自然科学基金资助(No:81202128、81272974、81071757、81172558)
高等学校博士学科点专项科研基金课题(No:20100162110036、20090162110065)
湖南省科技厅重点项目(No:2010TP4012-1)
中南大学自由探索计划青年教师助推基金(No:2012QNZT099)
福建省卫生厅医学创新课题(No:2012-cxb-44)
关键词
喉鳞状细胞癌
泛素
淋巴结转移
laryngeal squamous cell carcinoma
ubiquitin
lymph node metastasis
