摘要
目的研究B细胞淋巴瘤-2(Bcl-2)、半胱氨酸天冬氨酸蛋白酶-3(Caspase-3)在溃疡性结肠炎(UC)肠黏膜上皮细胞及固有层炎症细胞内的表达情况及其与UC分期的关系。方法选择UC活动期、缓解期及正常结肠黏膜组织(对照组)各25例,显微镜观察UC活动期、缓解期的病理形态学特征;采用免疫组织化学SP法检测各组标本上皮细胞和炎症细胞中Bcl-2、Caspase-3的表达情况;并与UC分期进行相关性分析。结果 3组肠黏膜上皮细胞中的Bcl-2、Caspase-3表达差异均无统计学意义;Bcl-2在固有层炎症细胞中表达为活动期组高于对照组(P<0.05),缓解期组与对照组差异无统计学意义;缓解期组的Caspase-3在固有层炎症细胞中的阳性表达级别较高。Bcl-2、Caspase-3与UC分期有关联性(r分别为0.371、0.445)。结论 Bcl-2、Caspase-3与UC的发病密切相关。检测Bcl-2、Caspase-3的表达水平对辅助UC分期具有一定应用价值。
Objective To study the correlation between the expressions of Bcl-2 and Caspase-3 in intestinal epithelial and inflammatory cells of lamina propria and the staging and pathogenesis of ulcerative colitis （UC）. Methods The pathomorphologieal changes of 25 samples of UC in the active phase and remission phase and 25 samples of control were observed under microscope. The expressions of Bcl-2 and Caspase-3 were detected by immunohistochemistry SP method. The correlations between expressions of Bcl-2, Caspase-3 and UC staging were analyzed. Results There were no significant dif- ferences in expressions of Bcl-2 and Caspase-3 in intestinal epithelial cells between three groups （P 〉 0.05）. The expression of Bel-2 in inflammatory cells of lamina propria was significantly higher in active UC group than that of normal control group （P 〈 0.05）, no significant difference between UC remission group and normal control group （P 〉 0.05）. There was a higher positive expression of Caspase-3 in inflammatory cells of lamina propria in UC remission group. There was correlation be- tween Bcl-2, Caspase-3 and UC staging （r= 0.371 and 0.4453 respectively）. Conclusion The expression levels of Bcl-2 and Caspase-3 were related with pathogenesis of UC. The detection of expression levels of Bcl-2 and Caspase-3 has a certain application value in UC staging.
出处
《天津医药》
CAS
北大核心
2013年第9期849-851,I0001,共4页
Tianjin Medical Journal
基金
广州市中医药中西医结合科研立项课题资助(项目编号2008B02)
